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TGGCGGCATTTTAACTTTCTTTAATGAATGCGGGCATATTTAATACGCGCTATGCGCATCGTATGCGAT-3' 1) What are the first five deoxyribonucleotides of the DNA template strand read by RNA polymerase in the 3' to 5' direction? 3'- -5' 2) What are the first five ribonucleotides of the MRNA transcript of this gene ? 5'- -3

TGGCGGCATTTTAACTTTCTTTAATGAATGCGGGCATATTTAATACGCGCTATGCGCATCGTATGCGAT-3' 1) What are the first five deoxyribonucleotides of the DNA template strand read by RNA polymerase in the 3' to 5' direction? 3'- -5' 2) What are the first five ribonucleotides of the MRNA transcript of this gene ? 5'- -3

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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A. Below is a small 2 exon long gene. The exons are underlined, and the 22 nucleotide long intron is the non-underlined sequence between the exons. TAG, TAA, and TGA are stop codons. 5'-TAGTGTATTGACATGATAGAAGCACTCACTATATTCTGACGTGCGACTATGCGTGGGGTTAGGT АТTGTGCTGACТTTТСТСAGGTGGCCCGTATAGGCТААGCTGCGСАТCGCCGCTAGTCGCTCAGTTCСGC TGGCGGCATTTTAACTTTCTTTAATGAATGCGGGCATATTTAATACCGCGCTATGCGCATCGTATGCGAT-3' 1) What are the first five deoxyribonucleotides of the DNA template strand read by RNA polymerase in the 3' to 5' direction? 3'- -5' 2) What are the first five ribonucleotides of the MRNA transcript of this gene ? 5'- -3' 3) What are the first five ribonucleotides following exon 1 in the mature MRNA transcript? 5'- -3' 4) What is the 5' UTR of the mature MRNA transcript in ribonucleotides? 5'- -3' 5A) What are the first five ribonucleotides of the 3' UTR? 5'- -3' B) What are the last five ribonucleotides of the 3' UTR? 5'- -3' 6) How many amino acids are in the protein encoded by the mRNA? amino acids 7) Using a codon chart, translate the mRNA transcript into protein, using the single letter amino acid code and no spaces. N terminus- -C terminus 8) The bolded "A" nucleotide near the beginning of line 2 is the adenine in the intron that is responsible for forming the lariat during splicing. It is thus part of the consensus splicing motif. A) If this is mutated to G, what are the 5 ribonucleotides following exon 1 in the mature MRNA transcript? 5'- -3' B) Does this mutation result in a frame shift? C) How many amino acids are in the protein encoded by the MRNA following this mutation? amino acids D) Using a codon chart, translate the MRNA transcript with the mutation described in 8A into protein, using the single letter amino acid code and no spaces. N terminus- -C terminus 9) In another mutation from the original wild-type sequence, the bolded G in the middle of the second line is mutated to A. A)How many amino acids are in the protein encoded by the mRNA? amino acids B) Using a codon chart (posted under the Translation module in Moodle), translate the mRNA transcript with the mutation described in 9 into protein, using the single letter amino acid code and no spaces. N terminus- -C terminus
Transcribed Image Text:A. Below is a small 2 exon long gene. The exons are underlined, and the 22 nucleotide long intron is the non-underlined sequence between the exons. TAG, TAA, and TGA are stop codons. 5'-TAGTGTATTGACATGATAGAAGCACTCACTATATTCTGACGTGCGACTATGCGTGGGGTTAGGT АТTGTGCTGACТTTТСТСAGGTGGCCCGTATAGGCТААGCTGCGСАТCGCCGCTAGTCGCTCAGTTCСGC TGGCGGCATTTTAACTTTCTTTAATGAATGCGGGCATATTTAATACCGCGCTATGCGCATCGTATGCGAT-3' 1) What are the first five deoxyribonucleotides of the DNA template strand read by RNA polymerase in the 3' to 5' direction? 3'- -5' 2) What are the first five ribonucleotides of the MRNA transcript of this gene ? 5'- -3' 3) What are the first five ribonucleotides following exon 1 in the mature MRNA transcript? 5'- -3' 4) What is the 5' UTR of the mature MRNA transcript in ribonucleotides? 5'- -3' 5A) What are the first five ribonucleotides of the 3' UTR? 5'- -3' B) What are the last five ribonucleotides of the 3' UTR? 5'- -3' 6) How many amino acids are in the protein encoded by the mRNA? amino acids 7) Using a codon chart, translate the mRNA transcript into protein, using the single letter amino acid code and no spaces. N terminus- -C terminus 8) The bolded "A" nucleotide near the beginning of line 2 is the adenine in the intron that is responsible for forming the lariat during splicing. It is thus part of the consensus splicing motif. A) If this is mutated to G, what are the 5 ribonucleotides following exon 1 in the mature MRNA transcript? 5'- -3' B) Does this mutation result in a frame shift? C) How many amino acids are in the protein encoded by the MRNA following this mutation? amino acids D) Using a codon chart, translate the MRNA transcript with the mutation described in 8A into protein, using the single letter amino acid code and no spaces. N terminus- -C terminus 9) In another mutation from the original wild-type sequence, the bolded G in the middle of the second line is mutated to A. A)How many amino acids are in the protein encoded by the mRNA? amino acids B) Using a codon chart (posted under the Translation module in Moodle), translate the mRNA transcript with the mutation described in 9 into protein, using the single letter amino acid code and no spaces. N terminus- -C terminus
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