Suppose you have a 11.4 cm long chromatography column with an inner diameter of 5.47 mm that is packed with a stationary phase that occupies 66.0% of the volume. What is the linear flow rate, ux, in centimeters per minute, if the volumetric flow rate, F, is 1.98 mL/min? Ux = cm/min How long will it take, in minutes, for the solvent to pass through the column? min tM = What is the retention time, in minutes, on this column for a solute with the retention factor, k, of 11.0? min tR =

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**Chromatography Column Analysis**

*Suppose you have a 11.4 cm long chromatography column with an inner diameter of 5.47 mm that is packed with a stationary phase that occupies 66.0% of the volume.*

**Problem 1: Linear Flow Rate Calculation**

- **Question:** What is the linear flow rate, \( u_x \), in centimeters per minute, if the volumetric flow rate, \( F \), is 1.98 mL/min?
  
  \[
  u_x = \_\_\_\_\_\_\_\_\_ \, \text{cm/min}
  \]

**Problem 2: Passage Time Calculation**

- **Question:** How long will it take, in minutes, for the solvent to pass through the column?
  
  \[
  t_M = \_\_\_\_\_\_\_\_\_ \, \text{min}
  \]

**Problem 3: Retention Time Calculation**

- **Question:** What is the retention time, in minutes, on this column for a solute with the retention factor, \( k \), of 11.0?

  \[
  t_R = \_\_\_\_\_\_\_\_\_ \, \text{min}
  \]

**Explanation:**
  
This exercise involves a series of calculations related to the physical and chemical properties of a chromatography column used for separating chemical compounds. Each question requires the application of specific formulas and understanding of the column's characteristics, such as length, diameter, and the proportion occupied by the stationary phase.
Transcribed Image Text:**Chromatography Column Analysis** *Suppose you have a 11.4 cm long chromatography column with an inner diameter of 5.47 mm that is packed with a stationary phase that occupies 66.0% of the volume.* **Problem 1: Linear Flow Rate Calculation** - **Question:** What is the linear flow rate, \( u_x \), in centimeters per minute, if the volumetric flow rate, \( F \), is 1.98 mL/min? \[ u_x = \_\_\_\_\_\_\_\_\_ \, \text{cm/min} \] **Problem 2: Passage Time Calculation** - **Question:** How long will it take, in minutes, for the solvent to pass through the column? \[ t_M = \_\_\_\_\_\_\_\_\_ \, \text{min} \] **Problem 3: Retention Time Calculation** - **Question:** What is the retention time, in minutes, on this column for a solute with the retention factor, \( k \), of 11.0? \[ t_R = \_\_\_\_\_\_\_\_\_ \, \text{min} \] **Explanation:** This exercise involves a series of calculations related to the physical and chemical properties of a chromatography column used for separating chemical compounds. Each question requires the application of specific formulas and understanding of the column's characteristics, such as length, diameter, and the proportion occupied by the stationary phase.
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