Preparing a Thin Layer Chromatography experiment there are some common mistakes. Explain what happens in this situation: Sample spots too big
Interpretation-
To tell about all the common mistakes when we prepare a thin layer chromatography (TLC) experiment. We tell about that mistakes especially when sample spots are too big
Introduction-
Thin layer chromatography (TLC ) is a experimental method where we analyzing the reactions mixtures by separating the compounds in the different type of solvent. This thin layer chromatography determine the different type of component in the mixture. It is use to identify the compounds and to check their purity also. TLC also use to monitor the progress of the reaction where we check how much reactant is left in the reaction mixture and how much product formation is take place .
TLC which generally consist three steps and that is spotting, development and visualization. and here we tell about why spots are too big by mistake so it is very important to take a very perfect spot on the TLC to run spot in a good manner.
Step by step
Solved in 2 steps
