Suppose you are trying to separate a mixture of compounds using reversed-phase liquid chromatography (RPLC). On the first run, using a mobile phase containing 40% tetrahydrofuran and 60% water, the peaks all elute between 0 and 3 min and are too close together to be quantitatively resolved. To improve the resolution of the peaks, should you increase or decrease the amount of tetrahydrofuran in the mobile phase for the next run? Incorrect. decrease increase The solvent tetrahydrofuran is less polar than water. In RPLC, a nonpolar stationary phase and a polar solvent is used for the separation. Eluent strength of the mobile phase increases as the polarity decreases. Decreasing the eluent strength of the mobile phase will result in an increase in the retention time and improved resolution of the compounds. not enough information In a separate experiment, you are trying to separate a mixture of compounds using normal-phase liquid chromatography (NPLC). On the first run, with a mobile phase containing 35% toluene and 65% acetonitrile, the peaks again eluted quickly and were poorly resolved. To improve the resolution of the peaks, should you increase or decrease the amount of acetonitrile in the mobile phase for the next run? decrease not enough information increase
Suppose you are trying to separate a mixture of compounds using reversed-phase liquid chromatography (RPLC). On the first run, using a mobile phase containing 40% tetrahydrofuran and 60% water, the peaks all elute between 0 and 3 min and are too close together to be quantitatively resolved. To improve the resolution of the peaks, should you increase or decrease the amount of tetrahydrofuran in the mobile phase for the next run? Incorrect. decrease increase The solvent tetrahydrofuran is less polar than water. In RPLC, a nonpolar stationary phase and a polar solvent is used for the separation. Eluent strength of the mobile phase increases as the polarity decreases. Decreasing the eluent strength of the mobile phase will result in an increase in the retention time and improved resolution of the compounds. not enough information In a separate experiment, you are trying to separate a mixture of compounds using normal-phase liquid chromatography (NPLC). On the first run, with a mobile phase containing 35% toluene and 65% acetonitrile, the peaks again eluted quickly and were poorly resolved. To improve the resolution of the peaks, should you increase or decrease the amount of acetonitrile in the mobile phase for the next run? decrease not enough information increase
Chemistry
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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Transcribed Image Text:Chemistry
Suppose you are trying to separate a mixture of compounds using reversed-phase liquid chromatography
(RPLC). On the first run, using a mobile phase containing 40% tetrahydrofuran and 60% water, the peaks all
elute between 0 and 3 min and are too close together to be quantitatively resolved. To improve the
resolution of the peaks, should you increase or decrease the amount of tetrahydrofuran in the mobile phase
for the next run?
Incorrect.
decrease
increase
The solvent tetrahydrofuran is less polar than water. In RPLC, a
nonpolar stationary phase and a polar solvent is used for the
separation. Eluent strength of the mobile phase increases as the
polarity decreases. Decreasing the eluent strength of the mobile
phase will result in an increase in the retention time and
improved resolution of the compounds.
not enough information
In a separate experiment, you are trying to separate a mixture of compounds using normal-phase liquid
chromatography (NPLC). On the first run, with a mobile phase containing 35% toluene and 65% acetonitrile,
the peaks again eluted quickly and were poorly resolved. To improve the resolution of the peaks, should
you increase or decrease the amount of acetonitrile in the mobile phase for the next run?
decrease
not enough information
increase
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