**Exercise 6: Analyzing Separation Effectiveness with Column Chromatography**In this exercise, you are tasked with separating two compounds using column chromatography. Upon running the column, you collect the eluent in five fractions. To evaluate the efficiency of your separation process, you analyze each fraction using Thin Layer Chromatography (TLC), as depicted in the diagram below.**TLC Plate Analysis:**The TLC plate consists of five lanes, each corresponding to a fraction obtained from the column chromatography:- **Lane 1:** Displays two distinct spots, indicating the presence of two compounds, suggesting incomplete separation.- **Lane 2:** Shows a single spot, likely one of the separated compounds.- **Lane 3:** Also displays a single spot, presumed to be the other compound.- **Lane 4:** Shows two spots, similar to Lane 1, indicating partial separation.- **Lane 5:** Displays two distinct spots, again suggesting incomplete separation.**Conclusion:**The TLC plate reveals that the separation of the two compounds was partially effective. Fractions 2 and 3 each contain only one compound, indicating some degree of success in separating them. However, the presence of two spots in fractions 1, 4, and 5 suggests that further optimization of the chromatography process is needed to achieve complete separation.

Column chromatography is a sub-class of liquid chromatography in which we have a solid as a…

6. Say you are trying to separate two compounds by column chromatography. You run a column and collect the eluent in five fractions. You analyze each fraction by TLC on the same plate (shown below). Based on the TLC plate, how effective was your separation? ••• ---1----1-- 1 2 3 4 5

6. Say you are trying to separate two compounds by column chromatography. You run a column and collect the eluent in five fractions. You analyze each fraction by TLC on the same plate (shown below). Based on the TLC plate, how effective was your separation? ••• ---1----1-- 1 2 3 4 5

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Question

**Exercise 6: Analyzing Separation Effectiveness with Column Chromatography**

In this exercise, you are tasked with separating two compounds using column chromatography. Upon running the column, you collect the eluent in five fractions. To evaluate the efficiency of your separation process, you analyze each fraction using Thin Layer Chromatography (TLC), as depicted in the diagram below.

**TLC Plate Analysis:**

The TLC plate consists of five lanes, each corresponding to a fraction obtained from the column chromatography:

- **Lane 1:** Displays two distinct spots, indicating the presence of two compounds, suggesting incomplete separation.
- **Lane 2:** Shows a single spot, likely one of the separated compounds.
- **Lane 3:** Also displays a single spot, presumed to be the other compound.
- **Lane 4:** Shows two spots, similar to Lane 1, indicating partial separation.
- **Lane 5:** Displays two distinct spots, again suggesting incomplete separation.

**Conclusion:**

The TLC plate reveals that the separation of the two compounds was partially effective. Fractions 2 and 3 each contain only one compound, indicating some degree of success in separating them. However, the presence of two spots in fractions 1, 4, and 5 suggests that further optimization of the chromatography process is needed to achieve complete separation.

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