This is the graph for gel filtration chromatography. Can you please explain why the graph has the maximum and minimum peak and relate it with the gel filtration concept such as the volume of elution, molecular weight, and so on. Thank you .

Macroscale and Microscale Organic Experiments
7th Edition
ISBN:9781305577190
Author:Kenneth L. Williamson, Katherine M. Masters
Publisher:Kenneth L. Williamson, Katherine M. Masters
Chapter9: Column Chromatography: Fluorenone, Cholesteryl Acetate, Acetylferrocene, And Plant Pigments
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This is the graph for gel filtration
chromatography. Can you please explain why
the graph has the maximum and minimum
peak and relate it with the gel filtration
concept such as the volume of elution,
molecular weight, and so on. Thank you .
Graph for Result 1: (Volume of elution (mL) with 1% bromophenol blue)
Volume of Elution (mL) vs Corrected OD
1.789
1.8
1.6
1.4
1.2
0.745
0.8
0.477
0.6
0.379.400.452
0.406
0.393
0.254
d28 0.25
0.4
9:128
0.161
0.115
0.2
0.060
2
6.
10
12
14
16
Volume of Elution (mL)
Corrected OD
Transcribed Image Text:This is the graph for gel filtration chromatography. Can you please explain why the graph has the maximum and minimum peak and relate it with the gel filtration concept such as the volume of elution, molecular weight, and so on. Thank you . Graph for Result 1: (Volume of elution (mL) with 1% bromophenol blue) Volume of Elution (mL) vs Corrected OD 1.789 1.8 1.6 1.4 1.2 0.745 0.8 0.477 0.6 0.379.400.452 0.406 0.393 0.254 d28 0.25 0.4 9:128 0.161 0.115 0.2 0.060 2 6. 10 12 14 16 Volume of Elution (mL) Corrected OD
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