Suh and Savizky studied the thermodynamics of human a - lactalbumin (HLA) unfolding in the absence (apo) or presence of various metal ions (Suh, J. J. and Savizky, R. M., Int. J. Biophys., 2011, 1(1), 1-6). You may assume that the protein starts out in the folded state and becomes denatured when it unfolds. According to the data below, which metal ion results in the greatest enthalpic stabilization of the folded state? AG (KJ/mol) -5.29 ± 0.81 4.10 ± 0.17 AS (kJ/mol*K) 0.61 ± 0.07 AH (kJ/mol) Experiment Аро-HLA MgCl; + apo-HLA ZNC1, + apo-HLA CaCl + apo-HLA CoCh + apo-HLA MnCl, + apo-HLA SICI, + apo-HLA CaCl + apo-HLA NaCl+ apo-HLA KC1+ apo-HLA 184.92 ± 20.78 174.05 ± 5.72 0.55 ± 0.02 121.84 ± 6.02 3.62 ± 0.13 0.38 ± 0.02 181.79 ± 11.56 6.06 ± 0.46 0.57±0.04 184.69 ± 10.47 10.68 ± 0.44 0.56 ± 0.03 238.65 ± 15.22 14.10 ± 0.88 0.72 ± 0.05 223.41 ± 10.61 15.08 ± 0.91 0.67 0.03 232.16 ± 14.87 19.91 ± 1.33 0.68 ± 0.04 162.17± 6.27 -4.19 ± 0.18 0.54 ± 0.02 154.02 ± 8.73 -3.58 ± 0.24 0.51 ± 0.03 Mn2+ Zn2+ K+ none of these - the enzyme is more stable in the absence of metal ions Co2+
Suh and Savizky studied the thermodynamics of human a - lactalbumin (HLA) unfolding in the absence (apo) or presence of various metal ions (Suh, J. J. and Savizky, R. M., Int. J. Biophys., 2011, 1(1), 1-6). You may assume that the protein starts out in the folded state and becomes denatured when it unfolds. According to the data below, which metal ion results in the greatest enthalpic stabilization of the folded state? AG (KJ/mol) -5.29 ± 0.81 4.10 ± 0.17 AS (kJ/mol*K) 0.61 ± 0.07 AH (kJ/mol) Experiment Аро-HLA MgCl; + apo-HLA ZNC1, + apo-HLA CaCl + apo-HLA CoCh + apo-HLA MnCl, + apo-HLA SICI, + apo-HLA CaCl + apo-HLA NaCl+ apo-HLA KC1+ apo-HLA 184.92 ± 20.78 174.05 ± 5.72 0.55 ± 0.02 121.84 ± 6.02 3.62 ± 0.13 0.38 ± 0.02 181.79 ± 11.56 6.06 ± 0.46 0.57±0.04 184.69 ± 10.47 10.68 ± 0.44 0.56 ± 0.03 238.65 ± 15.22 14.10 ± 0.88 0.72 ± 0.05 223.41 ± 10.61 15.08 ± 0.91 0.67 0.03 232.16 ± 14.87 19.91 ± 1.33 0.68 ± 0.04 162.17± 6.27 -4.19 ± 0.18 0.54 ± 0.02 154.02 ± 8.73 -3.58 ± 0.24 0.51 ± 0.03 Mn2+ Zn2+ K+ none of these - the enzyme is more stable in the absence of metal ions Co2+
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Transcribed Image Text:Suh and Savizky studied the thermodynamics of human a -
lactalbumin (HLA) unfolding in the absence (apo) or presence of
various metal ions (Suh, J. J. and Savizky, R. M., Int. J. Biophys., 2011,
1(1), 1-6). You may assume that the protein starts out in the folded
state and becomes denatured when it unfolds. According to the data
below, which metal ion results in the greatest enthalpic stabilization
of the folded state?
AS (kJ/mol*K)
0.61 ± 0.07
0.55 ± 0.02
AH (kJ/mol)
Experiment
Аро-HLA
MĘC1; + apo-HLA
ZnCl, + apo-HLA
CdCl, + apo-HLA
CoCh + apo-HLA
MnCl, + apo-HLA
SICI, + apo-HLA
CaCl + apo-HLA
- apo-HLA
KC1+ apo-HLA
AG (KJ/mol)
184.92 ± 20.78
-5.29 ± 0.81
4.10 ± 0.17
3.62 0.13
174.05 ± 5.72
121.84 ± 6.02
0.38 ± 0.02
181.79 ± 11.56
6.06 = 0.46
0.57 ±0.04
184.69 ± 10.47
10.68 ± 0.44
0.56 ± 0.03
238.65 ± 15.22
14.10 ± 0.88
0.72 ± 0.05
15.08 ± 0.91
19.91 ± 1.33
223.41 ± 10.61
0.67 +0.03
232.16 ± 14.87
0.68 0.04
NaCl+a
162.17 ± 6.27
-4.19 + 0.18
0.54 + 0.02
154.02 ± 8.73
-3.58 ± 0.24
0.51 + 0.03
Mn2+
Zn2+
K+
none of these - the enzyme is more stable in the absence of metal
ions
Со2+
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