Suh and Savizky studied the thermodynamics of human a - lactalbumin (HLA) unfolding in the absence (apo) or presence of various metal ions (Suh, J. J. and Savizky, R. M., Int. J. Biophys., 2011, 1(1), 1-6). You may assume that the protein starts out in the folded state and becomes denatured when it unfolds. According to the data below, which metal ion results in the greatest enthalpic stabilization of the folded state? AG (KJ/mol) -5.29 ± 0.81 4.10 ± 0.17 AS (kJ/mol*K) 0.61 ± 0.07 AH (kJ/mol) Experiment Аро-HLA MgCl; + apo-HLA ZNC1, + apo-HLA CaCl + apo-HLA CoCh + apo-HLA MnCl, + apo-HLA SICI, + apo-HLA CaCl + apo-HLA NaCl+ apo-HLA KC1+ apo-HLA 184.92 ± 20.78 174.05 ± 5.72 0.55 ± 0.02 121.84 ± 6.02 3.62 ± 0.13 0.38 ± 0.02 181.79 ± 11.56 6.06 ± 0.46 0.57±0.04 184.69 ± 10.47 10.68 ± 0.44 0.56 ± 0.03 238.65 ± 15.22 14.10 ± 0.88 0.72 ± 0.05 223.41 ± 10.61 15.08 ± 0.91 0.67 0.03 232.16 ± 14.87 19.91 ± 1.33 0.68 ± 0.04 162.17± 6.27 -4.19 ± 0.18 0.54 ± 0.02 154.02 ± 8.73 -3.58 ± 0.24 0.51 ± 0.03 Mn2+ Zn2+ K+ none of these - the enzyme is more stable in the absence of metal ions Co2+
Suh and Savizky studied the thermodynamics of human a - lactalbumin (HLA) unfolding in the absence (apo) or presence of various metal ions (Suh, J. J. and Savizky, R. M., Int. J. Biophys., 2011, 1(1), 1-6). You may assume that the protein starts out in the folded state and becomes denatured when it unfolds. According to the data below, which metal ion results in the greatest enthalpic stabilization of the folded state? AG (KJ/mol) -5.29 ± 0.81 4.10 ± 0.17 AS (kJ/mol*K) 0.61 ± 0.07 AH (kJ/mol) Experiment Аро-HLA MgCl; + apo-HLA ZNC1, + apo-HLA CaCl + apo-HLA CoCh + apo-HLA MnCl, + apo-HLA SICI, + apo-HLA CaCl + apo-HLA NaCl+ apo-HLA KC1+ apo-HLA 184.92 ± 20.78 174.05 ± 5.72 0.55 ± 0.02 121.84 ± 6.02 3.62 ± 0.13 0.38 ± 0.02 181.79 ± 11.56 6.06 ± 0.46 0.57±0.04 184.69 ± 10.47 10.68 ± 0.44 0.56 ± 0.03 238.65 ± 15.22 14.10 ± 0.88 0.72 ± 0.05 223.41 ± 10.61 15.08 ± 0.91 0.67 0.03 232.16 ± 14.87 19.91 ± 1.33 0.68 ± 0.04 162.17± 6.27 -4.19 ± 0.18 0.54 ± 0.02 154.02 ± 8.73 -3.58 ± 0.24 0.51 ± 0.03 Mn2+ Zn2+ K+ none of these - the enzyme is more stable in the absence of metal ions Co2+
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter30: Capillary Electrophoresis, Electrochromatography, And Field-flow Fractionation
Section: Chapter Questions
Problem 30.12QAP
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