Sickle-cell anemia arises from a mutation in the gene for the β chain of human hemoglobin. The change from GAG to GTG in the mutant eliminates a cleavage site for the restriction enzyme Mst II, which recognizes the target sequence CCTGAGG. These findings form the basis of a diagnostic test for the sickle-cell gene. Propose a rapid procedure for distinguishing between the normal and the mutant gene. Would a positive result prove that the mutant contains GTG in place of GAG?
Sickle-cell anemia arises from a mutation in the gene for the β chain of human hemoglobin. The change from GAG to GTG in the mutant eliminates a cleavage site for the restriction enzyme Mst II, which recognizes the target sequence CCTGAGG. These findings form the basis of a diagnostic test for the sickle-cell gene. Propose a rapid procedure for distinguishing between the normal and the mutant gene. Would a positive result prove that the mutant contains GTG in place of GAG?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Sickle-cell anemia arises from a mutation in the gene for the β chain of human hemoglobin. The change from GAG to GTG in the mutant eliminates a cleavage site for the restriction enzyme Mst II, which recognizes the target sequence CCTGAGG. These findings form the basis of a diagnostic test for the sickle-cell gene. Propose a rapid procedure for distinguishing between the normal and the mutant gene. Would a positive result prove that the mutant contains GTG in place of GAG?
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