SET 1: PEPTIDE SEQUENCING BY ENZYME DEGRADATION AND EDMANN TECHNIQUE A. Predict the fragments that will be generated from the treatment of the following peptide with: a.) trypsin b.) chymotrypsin c.) and S. aureus V8 protease G-A-W-R-N-A-K-E-F-G-Q WRITE the fragments in this table: trypsin chymotrypsin S. aureus V8 protease G-A-W-R; N-A-K; E-F-G-Q G-A-W; R-N-A-K-E-F; G-Q G-A-W-R-N; A-K-E; F-G-Q B. The sequence of a 29 aa long peptide can be determined from the following data: a. Treatment of the peptide with dansyl chloride reveals that the amino terminal is Val. b. Trypsin digestion, separation of peptides, and Edmann technique give the sequences for peptide fragments as follows: T1 V-G-A-H-A-G-E-Y-G-A-E-A-T-E C- Terminal Peptide fragment T2 A-A-W-G-K & T4 T-N-V-K middle peptide fragments T3 V-L-S-P-A-K N-Terminal Peptide fragment c. Chymotrypsin digestion, separation of peptides, and Edmann technique give the sequences for peptide fragments as follows: C1 G-A-E-A-T-E C- Terminal peptide fragment C2 G-K-V-G-A-H-A-G-E-Y Middle fragment C3 V-L-S-P-A-K-T-N-V-K-A-A-W N- Terminal peptide fragment What is the sequence for the peptide? Show your solution here: Label the fragments when reconstructing the peptide chain e.g., for trypsin T-1, T-2 etc. ANSWER THIS

SET 1: PEPTIDE SEQUENCING BY ENZYME DEGRADATION AND EDMANN TECHNIQUE A. Predict the fragments that will be generated from the treatment of the following peptide with: a.) trypsin b.) chymotrypsin c.) and S. aureus V8 protease G-A-W-R-N-A-K-E-F-G-Q WRITE the fragments in this table: trypsin chymotrypsin S. aureus V8 protease G-A-W-R; N-A-K; E-F-G-Q G-A-W; R-N-A-K-E-F; G-Q G-A-W-R-N; A-K-E; F-G-Q B. The sequence of a 29 aa long peptide can be determined from the following data: a. Treatment of the peptide with dansyl chloride reveals that the amino terminal is Val. b. Trypsin digestion, separation of peptides, and Edmann technique give the sequences for peptide fragments as follows: T1 V-G-A-H-A-G-E-Y-G-A-E-A-T-E C- Terminal Peptide fragment T2 A-A-W-G-K & T4 T-N-V-K middle peptide fragments T3 V-L-S-P-A-K N-Terminal Peptide fragment c. Chymotrypsin digestion, separation of peptides, and Edmann technique give the sequences for peptide fragments as follows: C1 G-A-E-A-T-E C- Terminal peptide fragment C2 G-K-V-G-A-H-A-G-E-Y Middle fragment C3 V-L-S-P-A-K-T-N-V-K-A-A-W N- Terminal peptide fragment What is the sequence for the peptide? Show your solution here: Label the fragments when reconstructing the peptide chain e.g., for trypsin T-1, T-2 etc. ANSWER THIS

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 7.8P

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Peptide Sequencing Answer numbers 1 and 2. For number 2, show the OVERLAP method.
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b. Cleavage with chymotrypsin produces the following fragments: Band A: CN , NLQY, GIVEQCCHKRSEY Band B: F, Y, DPTKM, IACCVRGF, RTTGHLCGKDLVNALY Cleavage with Staphilococcus aureus V8 protease produces the following fragments: Band A: GIVE, YNLQNYCN, QCCHKRCSE Band B: PTKM, RTTGHLCGKD, LVNALYIACGVRGFFYD What is the amino acid sequence of the protein? Type your response
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