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A student conducted an experiment on protein isolation from mungbean. After isolating the protein, the student characterized the isolate using the Bradford assay. Standard solutions of bovine serum albumin (BSA) were prepared and 0.1 mL of each solution was subjected to Bradford assay. Absorbance readings were made at 595 nm and summarized below. Concentration of Absorbance at BSA (mg/mL) 595 nm 1.2 1.12 1.0 0.97 0.8 0.79 0.6 0.59 0.4 0.37 0.2 0.17 The student found out that 0.1 ml sample of the protein extract from mungbean gave an absorbance of 0.83 in the same assay. 1. How much was the concentration of the protein in the mungbean sample (in mg/mL)? Show all equations and calculations. 2. Draw a graph for the readings of the standard solutions and absorbances. Indicate the approximate location of the calculated concentration of the mungbean sample in your graph.

A student conducted an experiment on protein isolation from mungbean. After isolating the protein, the student characterized the isolate using the Bradford assay. Standard solutions of bovine serum albumin (BSA) were prepared and 0.1 mL of each solution was subjected to Bradford assay. Absorbance readings were made at 595 nm and summarized below. Concentration of Absorbance at BSA (mg/mL) 595 nm 1.2 1.12 1.0 0.97 0.8 0.79 0.6 0.59 0.4 0.37 0.2 0.17 The student found out that 0.1 ml sample of the protein extract from mungbean gave an absorbance of 0.83 in the same assay. 1. How much was the concentration of the protein in the mungbean sample (in mg/mL)? Show all equations and calculations. 2. Draw a graph for the readings of the standard solutions and absorbances. Indicate the approximate location of the calculated concentration of the mungbean sample in your graph.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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A student conducted an experiment on protein isolation from mungbean. After isolating the protein, the student characterized the isolate using the Bradford assay. Standard solutions of bovine serum albumin (BSA) were prepared and 0.1 ml of each solution was subjected to Bradford assay. Absorbance readings were made at 595 nm and summarized below. Concentration of Absorbance at BSA (mg/mL) 595 nm 1.2 1.12 1.0 0.97 0.8 0.79 0.6 0.59 0.4 0.37 0.2 0.17 The student found out that 0.1 mL sample of the protein extract from mungbean gave an absorbance of 0.83 in the same assay. 1. How much was the concentration of the protein in the mungbean sample (in mg/mL)? Show all equations and calculations. 2. Draw a graph for the readings of the standard solutions and absorbances. Indicate the approximate location of the calculated concentration of the mungbean sample in your graph.
Transcribed Image Text:A student conducted an experiment on protein isolation from mungbean. After isolating the protein, the student characterized the isolate using the Bradford assay. Standard solutions of bovine serum albumin (BSA) were prepared and 0.1 ml of each solution was subjected to Bradford assay. Absorbance readings were made at 595 nm and summarized below. Concentration of Absorbance at BSA (mg/mL) 595 nm 1.2 1.12 1.0 0.97 0.8 0.79 0.6 0.59 0.4 0.37 0.2 0.17 The student found out that 0.1 mL sample of the protein extract from mungbean gave an absorbance of 0.83 in the same assay. 1. How much was the concentration of the protein in the mungbean sample (in mg/mL)? Show all equations and calculations. 2. Draw a graph for the readings of the standard solutions and absorbances. Indicate the approximate location of the calculated concentration of the mungbean sample in your graph.
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