Select ALL that apply. An organism that can undergo fermentation could possibly be an Obligate Aerobe Obligate Anaerobe Facultative anaerobe Aerotolerant anaerobe Select the things that are NOT performed when observing motility under the microscope Stain is applied to see the cells properly The oil immersion lens is used for maximum magnification The slide is heat-fixed The condenser and iris diaphragm are adjusted to enhance the appearance of the cells The four biochemical tests that help to identify members of the family Enterobacteriaceae is referred to as Methyl Red ViC test IMViC test Voges Proskauer test MIViC test Which of the following statements about preparing a Hanging Drop slide is true? Petroleum jelly is mixed with the culture to slow down the cell movement The drop of culture does not touch the cavity slide, only the cover slip. The cavity of the cavity slide is filled with sample A thread is used to hang the drop from the cover slip If we see a red colour filling the entire tube of motility media, this indicates? The oxygen gradient has not properly formed. The tube is has been exposed to oxygen The bacteria have not spread through the media yet The bacteria have spread and grown throughout the entire media Which of the following statements is NOT TRUE fermentation releases less energy than respiration oxygen is necessary for fermentation to breakdown sugars to test for fermentation, bacteria can be inoculated into a media which contains a particular sugar in the lab procedure for testing carbohydrate fermentation, lactose broth and glucose broth were used Which of the following statements is NOT TRUE fermentation takes place in an anaerobic environment to test for fermentation, bacteria can be inoculated into a media which contains a particular sugar in the lab procedure for testing carbohydrate fermentation, fructose broth and mannitol broth were used fermentation releases less energy than respiration Which of the following statements is NOT TRUE after inoculating with the test bacteria, the media is kept in the incubator at room temperature the enzyme urease hydrolyzes urea and provides a supply of nitrogen for the bacteria the citrate test determines whether or not bacteria are capable of using citrate as the only carbon source phenol red is included in the media as the pH indicator for the urease test Which of the following statements is TRUE the methyl red (MR) test detects acid production after incubation, 5 drops of Kovac's reagent is added to complete the MR test for the MR test, a positive reaction is indicated by the development of a yellow colour the methyl red (MR) test detects the formation of end products that have a neutral pH Why does motility media contain a lower amount of agar than ordinary agar? We cannot normally see through regular media It makes it easier to puncture with the inoculation needle Bacteria can still swim through it if they are motile because it is more "loose" It allows air to penetrate so that aerobic cultures can survive
Select ALL that apply. An organism that can undergo fermentation could possibly be an
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Obligate Aerobe |
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Obligate Anaerobe |
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Facultative anaerobe |
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Aerotolerant anaerobe |
Select the things that are NOT performed when observing motility under the microscope
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Stain is applied to see the cells properly |
||
|
The oil immersion lens is used for maximum magnification |
||
|
The slide is heat-fixed |
||
|
The condenser and iris diaphragm are adjusted to enhance the appearance of the cells |
The four biochemical tests that help to identify members of the family Enterobacteriaceae is referred to as
|
Methyl Red ViC test |
||
|
IMViC test |
||
|
Voges Proskauer test |
||
|
MIViC test |
Which of the following statements about preparing a Hanging Drop slide is true?
|
Petroleum jelly is mixed with the culture to slow down the cell movement |
||
|
The drop of culture does not touch the cavity slide, only the cover slip. |
||
|
The cavity of the cavity slide is filled with sample |
||
|
A thread is used to hang the drop from the cover slip |
If we see a red colour filling the entire tube of motility media, this indicates?
|
The oxygen gradient has not properly formed. |
||
|
The tube is has been exposed to oxygen |
||
|
The bacteria have not spread through the media yet |
||
|
The bacteria have spread and grown throughout the entire media |
Which of the following statements is NOT TRUE
|
fermentation releases less energy than respiration |
||
|
oxygen is necessary for fermentation to breakdown sugars |
||
|
to test for fermentation, bacteria can be inoculated into a media which contains a particular sugar |
||
|
in the lab procedure for testing carbohydrate fermentation, lactose broth and glucose broth were used |
Which of the following statements is NOT TRUE
|
fermentation takes place in an anaerobic environment |
||
|
to test for fermentation, bacteria can be inoculated into a media which contains a particular sugar |
||
|
in the lab procedure for testing carbohydrate fermentation, fructose broth and mannitol broth were used |
||
|
fermentation releases less energy than respiration |
Which of the following statements is NOT TRUE
|
after inoculating with the test bacteria, the media is kept in the incubator at room temperature |
||
|
the enzyme urease hydrolyzes urea and provides a supply of nitrogen for the bacteria |
||
|
the citrate test determines whether or not bacteria are capable of using citrate as the only carbon source |
||
|
phenol red is included in the media as the pH indicator for the urease test |
Which of the following statements is TRUE
|
the methyl red (MR) test detects acid production |
||
|
after incubation, 5 drops of Kovac's reagent is added to complete the MR test |
||
|
for the MR test, a positive reaction is indicated by the development of a yellow colour |
||
|
the methyl red (MR) test detects the formation of end products that have a neutral pH |
Why does motility media contain a lower amount of agar than ordinary agar?
|
We cannot normally see through regular media |
||
|
It makes it easier to puncture with the inoculation needle |
||
|
Bacteria can still swim through it if they are motile because it is more "loose" |
||
|
It allows air to penetrate so that aerobic cultures can survive |
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