Laboratory Exercises in Microbiology Results for Metabolic Tests McLaughlin and Petersen Lactose Bacteria Gram stain Morphology Arrangement Fermentation Gelatinase Amylase Urease Citrate permease Tryptophanase (Indole test) Catalase (Simmon's citrate) Bacillus subtilis Corynebacterium xerosis Enterobacter aerogenes Escherichia coli Micrococcus luteus Pseudomonas aeruginosa Proteus vulgaris Staphylococcus аureus Streptococcus faecalis Serratia тarcescens The tests in the table above will be available for your practical exam. Use your results from Lab 5 to fill out this table. Results for the bacteria that were not tested in Lab 5 can be found on the Microbiology Review site on Blackboard.

Q: Create a dichotomous key Use the following bacteria and place them at the top of your key:…

A: Use the following bacteria and place them at the top of your key: Enterococcus faecalis…

Q: Chose the right answer

A: The correct option is E . Cyclospora cayetanensis

Q: The following is a picture of Eosin Methylene Blue Agar. What does the growth on this plate…

A: Eosin Methylene Blue (EMB) agar is a selective and differential culture medium which selectively…

Q: Based on the data below, how much more UV resistant was B. licheniformis relative to S. aureus? (All…

A: UV radiation cause production of large number of ROS and also get absorbed into DNA thus leads to…

Q: Help indetifying the unknown bacteria ? Gram Stain: Purple Cell Morpholgy: Rods Oxygen…

A: Bacteria are prokaryotic, simple, unicellular, microscopic organisms that are devoid of membrane…

Q: What is an endospore?

A: There are various means which is used to kill the bacteria and other organisms. The methods used can…

Q: Sanger spectrophotometer Coulter counter plate counts centrifugation direct Petroff-Hauser turbidity…

A: Microbiology is the study of the biology of microscopic organisms - viruses, bacteria, algae, fungi,…

Q: What additional test would you need to perform to distinguish between the two organisms you have…

A: Providencia species, P. alcalifaciens and P. rettgeri show similar results in biochemical tests.…

Q: Using the workflow images and data table match. the results of each te Not determined by the…

A: Staphylococcus aureus is a gram-positive bacterium that causes a number of clinical conditions. This…

Q: earch for the following bacteria and complete the table below. Bacterium Stain Description or…

A: Flavobacterium capsulatum when stained with capsule stain shows a Pericellular Halo around the cell…

Q: Discuss the importance of some bacteria to undergo starvation during stationary phase and importance…

A: The importance of some bacteria to undergo starvation during stationary phase is that : In…

Q: Help indetifying the unknown bacteria ? Gram Stain: Pink  Cell Morpholgy: Bacillus  Oxygen…

A: Bacteria are prokaryotic unicellular organism that are devoid of membrane bound organelles and…

Q: Organism Characteristic Result Pseudomonas fluorescens Oxidase Escherichia coli VP Enterobacter…

A: The IMViC test includes four different tests that are used to identify and differentiate between the…

Q: Organism - Enterobacter aerogenes 1. 1. Every organism is unique! Provide some interesting facts or…

A: Bacteria are organisms not visible to the naked eyes that are found in almost every part of the…

Q: Which microbe usually initiates a biofilm on our teeth? O Staphylococcus aureus O Bacillus anthracis…

A: Biofilm is the growth of one or more microorganisms on a surface. Teeth plaque is one kind of…

Q: Please answer yes ✓ or not X

A: The differential nature of the Gram stain is predicated on the power of some microorganism cells to…

Q: O Yes O No

A: Growing bacteria on a petri dish is a fundamental technique used in microbiology to culture and…

Q: Which of the following metabolic tests would only be performed on Gram-positive bacillus?  VP TSIA…

A: The objective of the question is to identify which among the given metabolic tests is specifically…

Q: An 89 year old man presented to a community hospital emergency room with weakness and inability to…

A: Bacteria are simple, microscopic prokaryotes that lack a nucleus. Cell walls are composed of…

Q: Ingredients Вef Yeast Definitions Agar Broth What does not grow What grows Chemoheterotrophs…

A: Since you have posted a question with multiple sub=parts, we will solve first three sub-parts for…

Q: Bacterium Z had the lab results below. What can you tell me about the protein metabolism of this…

A: Bacteria are much the same as other life forms so far as proteins are concerned — they contain…

Q: Bacteria of GI Tract Lab. 1. What information can you accurately report about this unknown sample

A: Different media plates help to characterise the type of organism.

Q: can you draw the following on positive simple staining in 1000x objective? thank you.…

A: For the purpose of staining, dyes are selected based on their chemical properties and the specimen…

Q: МАС EMB Agar НЕА SSA XLD Agar Escherichia coli Klebsiella pneumoniae Citrobacter freundii Serratia…

A:

Q: What color would Staphylococcus aureus cells be with the endospore stain shown in the photo?

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: Which lactose (+), Gram negative, short rod forms a pink to brick red colony on MacConkey's agar and…

A: Bacteria that cannot retain the crystal violet stain as their cell envelopes are composed of…

Q: McConkey agar plate is used to determine     which carbohydrates a bacteria can ferment     if the…

A: To grow bacteria, required nutrients should be incorporated into the culture media. These culture…

Q: Are any two the same species?

A: Introduction: Bacteria are single-celled bacteria that can be found practically anywhere on Earth,…

Q: mention any three fluids used in cleaning lense in the microbiology laboratory

A: A laboratory tool called a microscope is used to look at things that are too tiny to be seen with…

Q: Question 45 Which of the following causes or rabbit fever and is classified as Class A bioterrorism…

A: Bioterrorism is the released biological agent intentionally to cause disease among large group of…

Q: B D F A с E Shape Flagella Staphylococc Bacillus Staphylococci Bacillus Diplobacillus Gram stain…

A: Introduction R. H. Whittaker’s classification system divides organisms into five major groups. These…

Q: (Match the phrases from column (A) with phrases in column (B) Anima SASP Axial Asbestos Proteins…

A: The individual form of life is called as the organism. Every organisms consist of some specific…

Q: Below are photos of 3 bacteria, letter lableled, inoculated onto MSA plates and incubated. Note no…

A: Microbiology is the study of microscopic organisms that are mostly not visible to the naked eye.…

Q: Which of these bacterial characteristics could be evaluated with one of the rapid identification…

A: Arabinose Fermentation Test is used to determine the if the microbes can ferment the carbohydrate…

Q: Test Description Terminal Terminal Medium, Incubation Incubation Results temperature time (°C)…

A: A difference in the physiology of bacteria from one to another is the necessity to utilize…

Q: Which of these microbial structures is the most difficult to kill when attempting to sterilize…

A: Answer of the question given below...

Q: Identify what microorganisms are in each of the 10 plates from studying the gram stain and agar…

A: Plate-1  The organism appears purple in the Gram staining. So, the organism is Gram-positive. The…

Q: Name of Organism megaterium pacter aerogenes chia coli occus luteus acterium smegmatis ria subflava…

A: Answer :: The above list contains both gram negative and gram positive bacteria. Biochemicals tests…

Q: Which of the following media selects for gram negative bacteria by inhibiting the growth of gram…

A: Selective media:- it usually allow the growth of a particular microbe and inhibit the growth of…

Q: Using the flowchart on Page 541 of your text, correctly identify the bacteria based of the…

A: Enterobacter are the Gram negative, oxidase negative, catalase positive and rod shaped (bacillus)…

Q: Which cellular structure is being detected in the motility test? bacterial ribosomes bacterial cilia…

A: Prokaryotes are characterised by the absence of nucleus and membrane bound organelles.

Q: Microorganism that has the urease enzyme present a O red (or pink) O yellow (or peach) color in the…

A: The Urease test had been developed by the microbiologist Christensen. This test is used particularly…

Q: Based on the pictures provided, tell me what I have for the gram-positive, and what I have for the…

A: A gram stain is a type of differential stain, which means it allows the distinction of one cell from…

Q: What type of microbes can not be effectively killed by low temperature (i.e. freezing) treatment?…

A: The organisms which can survive in extreme conditions are known as archaebacteria.

Q: test a efficient test for a unkown intestinal bacteria.

A: Starch hydrolysis test is a testing method which is used to identify bacteria that can hydrolyze…

Q: Complete the data table. Table 2. Eosin Methylene Blue Agar Bacterium Result Enterobacter aerogenes…

A: INTRODUCTION Enterobacter aerogenes Klebsiella aerogenes is the organism that is also known as…

Q: MICROCOCCI gram positive cocci in strict aerobe V on BAP

A: The micrococcii are strict aerobes they are gram positive and appear purple colour colonies in gram…

Q: Gram classification Etiologic agent Drug (s) of choice Vaccines (if applicable) Gram-positive cocci…

A: Gram classification  Etiological agent   DRUGS OF CHOICE  VACCINE  Gram positive cocci…

Q: Which of the following organisms is most closely related?

A: Introduction: Bacteria are single-celled bacteria that can be found practically anywhere on Earth,…

Q: Bacterium F had the lab results below. What can you tell me about the carbon food sources it…

A: The citrate test is one of the commonly performed metabolic tests to detect the ability of the…

Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.