Sanger spectrophotometer Coulter counter plate counts centrifugation direct Petroff-Hauser turbidity color most probable number flow cytometer colony forming units filtration The most obvious way to measure microbial numbers is using a microscope for counts. A special slide called a known volume of culture. counting chamber is used to count cells in a Cells can be stained and then counted by interrupting a laser beam as they pass through a Another machine for counting cells, the through a pore change electrical currents. Viable counting methods often use on the surface of agar plates. detects when cells moving where diluted cultures are spread Cells from very dilute samples are often concentrated by plating. before Each cell spread on the plates that produces a colony is counted and the number reported as While plating techniques are valuable another viable counting method, analysis, can be used when cells can not be cultured on solid media. A simple but indirect assay of culture density uses a spectrophotometer to measure

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Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.