Table 3 - Determination Tube # Potato Expt. Temp. 2c 3c 4c 5c extract (mL) 2 2 2 2 room temp 20 °℃ 40 °℃ 75 °℃ Boiling 100 °C of the optimum temperature of catechol oxidase enzyme. 1st Absorbance 0 min. at Expt. Temp. dH₂O Catechol (mL) (mL) 0 0 0 0 13 13 13 13 Start Time: 4:20 Absorbance: 0.072 Start Time: 4:25 Absorbance: 0.114 Start Time: 4:25 Absorbance: 2nd Absorbance after 10 min. in Expt Temp. Absorbance: 0.097 Time for reading: 4:30 Absorbance: 0.128 Time for reading: 4:33 Absorbance: Subtract 1st from 2nd absorbance 0.056 0.137 Time for reading: 4:35 Absorbance: 0.132 0-193 Start Time: 4:27 Time for reading: 437 Absorbance: 0-126 0.023 0.061 0.029 3rd Absorbance after 10 more min. at Room Temp. Time for reading: 4:40 Absorbance: Based on the data from Table 2 answer these questions: Q10) What is the enzyme's optimum temperature? Answer: Q11) Use the difference between 2nd and 1st absorbance to support your conclusion for the optimum temperature. 0.159 Time for reading: 4:43 Absorbance: 0.119 Time for reading: 4:45 Absorbance: Subtract 2nd from 3rd absorbance 0-209 Time for reading: 4:47 Absorbance: 0.115 0.031 -0.018 0.016 -0.011

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