Samples Absorbance undiluted 0.715 1:4 dilution ratio 0.447 1:9 dilution ratio 0.321 a. Calculate the slope, y-intercept, and linearity constant for the correct absorbance vs, concentration linear plot. b. Determine the actual or original protein concentration of the sample in ug/mL.

Samples Absorbance undiluted 0.715 1:4 dilution ratio 0.447 1:9 dilution ratio 0.321 a. Calculate the slope, y-intercept, and linearity constant for the correct absorbance vs, concentration linear plot. b. Determine the actual or original protein concentration of the sample in ug/mL.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

y biochemical calculation w linear regression parameters. please write complete values and answer all

Samples
Absorbance
undiluted
0.715
1:4 dilution ratio
0.447
1:9 dilution ratio
0.321
a. Calculate the slope, y-intercept, and linearity constant for the correct
absorbance vs, concentration linear plot.
b. Determine the actual or original protein concentration of the sample in
ug/mL.

6) The protein content of an unknown sample was determined using bovine
serum albumin (BSA) as standard. The absorbance values of the standard
solutions and of the samples are as follows.
BSA concentration
Absorbance
(ug/mL)
0.005
0.00
0.063
0.20
0.121
0.40
0.276
0.60
0.336
0.80
0.406
1.00
0.449
1.20

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