all224 811 468 61+Time left •;•oQuestion IPNot yet answeredMarked out of •.IFYFlag questionWhat would be the effect on the PCR reactionif any of the following circumstances arose: 1)there are no primers in the reaction, Y) thereare no DNTPS in the reaction, P") there is noTaq polymerase in the reaction?a.The reaction will cease after a few cyclesb.The PCR reaction will not commenceO c.PCR would proceed normallyd.Non-specific PCR of random templates willoccur

Polymerase Chain Reaction is a laboratory technique to make millions of copies of a particular…

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Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

In PCR amplification why is it important to have a negative control?
Consider the following DNA sample: 5' CCGAGAACTTA 3' Draw a gel showing the following: 1. Sanger sequencing (ddNTP's): a. Lane 1: A pre-made ladder with the following molecular weights: 2, 5, and 9 b.p. Draw and arrow and label each band for your ladder. b. Lane 2: PCR of sample using dGTP, dATP, dCTP, and dTTP c. Lane 3: PCR of sample using ddGTP, dATP, dCTP, and dTTP d. Lane 4: PCR of sample using dGTP, ddATP, dCTP, and dTTP e. Lane 5: PCR of sample using ddGTP, ddATP, ddCTP, and ddTTP
1. Benedicts test is used to identify reducing sugars. What is the correct principle? A. Heating of simple carbohydrate is 5. A technique in genetic testing in which DNA fragments are amplified to more useful. added with Copper (II) forming copper (1) oxide which is an orange/ brick red complex. A. Restriction endonucleases B. Cloning C. Probes D. Southern blotting B. Polviodide ions is added to carbohydrate forming a black colored absorption complex, dextrin. C. Carbohydrate is added to H2504 6. The wrong monosaccharide combination undergoing dehydration and creating is. A. Sucrose = Fructose + galactose B. Lactose = galactese + glucose C. Maltose = Glucose + glucose D. All of the above. furfural which is combined with alpha naphtol creating a purple colored complex. D. None of the above. 7. Arrange the correct sequence in southern 2. What reagent is added to a sugar- jovertass solution in order to quantify its reaction seectrophotometrically? A. Benedict's solution. B. Biuret's…
You are tasked with amplifying a gene of interest with PCR. a. After performing a Southern blot, you see no signal on your gel. What could have happened with your PCR? b. After troubleshooting your PCR, you now want to perform Reverse Transcriptase (RT) PCR to look at MRNA expression. Assuming the primers in 2a were not the problem for your DNA and using the same primers as 2a, you see no signal on your Southern blot. What are possible causes?
What are the reasons why there needs to be more than 10 cycles in the PCR process? Name 2 reasons.
Which of the following reagents is NOT correct reagents for the Sanger method of DNA sequencing? a. All four dNTPs b. a small amount of specific ddNTP c. DNA Polymerase d. DNA template to be sequenced e. Pair of primers
1. Introduction: Define the following concepts and processes 1) List material used in a PCR reaction 2) List steps of PCR reaction and temperatures for each step
What is the principle of the SNP (single nucleotide polymorphisms) in the diagnosis of human diseases? a. PCR product of a gene is different from the expected one b. The size of a recombinant DNA is different from the expected one c. Mutation of a single base in a gene makes the size of a band digested by specific restriction enzymes different from the expected one d. The DNA band detected by Southern blot is different from that by Northern blot
What is the final volume of the individual PCR reactions we are making?
8) PCR (polymerase chain reaction) 9) plasmid A. Creates millions of copies of DNA within hours B. Used to test for the presence of hundreds to thousands of active genes at the same tim C. Used as a vector to replicate foreign DNA inside bacteria D. Able to cut double stranded DNA at almost any desired location for genome editing Which of the following is not an example of RNA processing. the addition of the poly-A tail to mRNA in the nucleus spliceosomes cutting mRNA in the nucleus the addition of the 3' cap to mRNA in the nucleus the joining together of exons in the nucleus
Think about the function of the primers in a PCR that is designed to amplify a specific sequence, and about the temperatures of the PCR cycle. After considering these two points, give two reasons why 4-nucleotide long primers are not appropriate for PCR and explain.
For DNA amplification using PCR to occur, which of the following are needed? A. DNA primers B. thermo stable DNA polymerase C. Helicase D. Choices A and B

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