all224 811 468 61+Time left •;•oQuestion IPNot yet answeredMarked out of •.IFYFlag questionWhat would be the effect on the PCR reactionif any of the following circumstances arose: 1)there are no primers in the reaction, Y) thereare no DNTPS in the reaction, P") there is noTaq polymerase in the reaction?a.The reaction will cease after a few cyclesb.The PCR reaction will not commenceO c.PCR would proceed normallyd.Non-specific PCR of random templates willoccur

Polymerase Chain Reaction is a laboratory technique to make millions of copies of a particular…

Answered: What would be the effect on the PCR…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

In PCR amplification why is it important to have a negative control?
What would be the outcome if the primers used in a polymerase chain reaction have lower GC content (<40 %), shorter, and more variable than the intended oligonucleotide sequence? a.The PCR reaction will cease after the first cycle b.The reaction will yield a mixture of non-specific products. c.All of these d.The PCR reaction will not start. e.The reaction will yield a single short PCR product.
Most PCR reactions do not use the more expensive types of DNA polymerase, which have DNA proofreading. How might this be a problem in accurately copying specific DNA sequences of the target gene?
Consider the following DNA sample: 5' CCGAGAACTTA 3' Draw a gel showing the following: 1. Sanger sequencing (ddNTP's): a. Lane 1: A pre-made ladder with the following molecular weights: 2, 5, and 9 b.p. Draw and arrow and label each band for your ladder. b. Lane 2: PCR of sample using dGTP, dATP, dCTP, and dTTP c. Lane 3: PCR of sample using ddGTP, dATP, dCTP, and dTTP d. Lane 4: PCR of sample using dGTP, ddATP, dCTP, and dTTP e. Lane 5: PCR of sample using ddGTP, ddATP, ddCTP, and ddTTP
What are the different types of PCR and their product concepts? Site and explain the comparison briefly.
1. Benedicts test is used to identify reducing sugars. What is the correct principle? A. Heating of simple carbohydrate is 5. A technique in genetic testing in which DNA fragments are amplified to more useful. added with Copper (II) forming copper (1) oxide which is an orange/ brick red complex. A. Restriction endonucleases B. Cloning C. Probes D. Southern blotting B. Polviodide ions is added to carbohydrate forming a black colored absorption complex, dextrin. C. Carbohydrate is added to H2504 6. The wrong monosaccharide combination undergoing dehydration and creating is. A. Sucrose = Fructose + galactose B. Lactose = galactese + glucose C. Maltose = Glucose + glucose D. All of the above. furfural which is combined with alpha naphtol creating a purple colored complex. D. None of the above. 7. Arrange the correct sequence in southern 2. What reagent is added to a sugar- jovertass solution in order to quantify its reaction seectrophotometrically? A. Benedict's solution. B. Biuret's…
You are tasked with amplifying a gene of interest with PCR. a. After performing a Southern blot, you see no signal on your gel. What could have happened with your PCR? b. After troubleshooting your PCR, you now want to perform Reverse Transcriptase (RT) PCR to look at MRNA expression. Assuming the primers in 2a were not the problem for your DNA and using the same primers as 2a, you see no signal on your Southern blot. What are possible causes?
they decided to check which bacterial colonies contains the recombinant gene W by PCR. they designed one set of primers, gene W forward, and gene W reverse, to amplify gene W. Complete the PCR profile that will be used below.
What are the reasons why there needs to be more than 10 cycles in the PCR process? Name 2 reasons.
All are true about PCR except A. Automated PCR machine called a thermal cycler B. A thermostable DNA polymerase is required C. Primer extension occurs at 72˚C D. Annealing involves binding of primer between 90-98˚C
Which of the following best describes the complete sequence of steps occurring during every cycle of PCR? I-The primers hybridize to the target DNA. II-The mixture is heated to a high temperature to denature the double stranded target DNA. III-Fresh DNA polymerase is added. IV-DNA polymerase extends the primers to make a copy of the target DNA. A) II, I, IV. B) I, III, II, IV. C) III, IV, I, II. D) III, IV, II. E) II, III, IV.
What is the purpose of adding a primer to a PCR reaction? Is this primer made up of DNA or RNA nucleotides? Explain your reasoning.

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