) Sam wants to alter his interrupted mating experiment by placing a filter in between the donor and the recipient cells in order to separate the two. Is Sam’s suggestion for this modification to the procedure utilized in interrupted mating experiments valid? Explain.
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
a) Sam wants to alter his interrupted mating experiment by placing a filter in between the donor and the recipient cells in order to separate the two. Is Sam’s suggestion for this modification to the procedure utilized in interrupted mating experiments valid? Explain.
b) Much to the dismay of the researcher with whom Sam is working, Sam is not only forgetful, but he is also lazy! Rather than devoting the necessary hours to conducting his assigned interrupted mating experiments, Sam has decided that his time would be better spent modifying the F plasmid so that the experiments wouldn’t require quite so much time. The diagram below shows a map of the E. coli F plasmid, similar to that shown in lecture. Sam is determined to “get rid of some of the excess DNA” present in the plasmid. Please keep in mind that the PI in the lab in which Sam works utilizes these F plasmids for interrupted mating experiments between Hfr and F- cells as well as for interrupted mating experiments involving F+ and F- cells.
c) Sam suggests that the region of the F plasmid that includes IS2, IS3, and Tn1000 be deleted. Good idea? Please EXPLAIN your answer.
- Alternatively, Sam suggests that the region labeled “transfer region” be deleted from the F plasmid, since it occupies so much of the F plasmid genome. Good idea? Please EXPLAIN your answer.
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