RNA sequence. ate 3' and 5' ends on BOTH strands ate which strand served as the TEMPLATE strand and which ING strand

Human Anatomy & Physiology (11th Edition)
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**Using the mRNA Sequence Analysis**

The task involves analyzing the given mRNA sequence: 

5’-GUCAGAUAUCAUGUAGGCUUAAGCCGUAGCAC-3’

### Objective

To determine the original double-stranded DNA sequence used to synthesize the above mRNA sequence and indicate specifics regarding the DNA strands.

### Instructions

1. **Write out the Double-Stranded DNA Sequence:**
   - **mRNA Sequence:** 5’-GUCAGAUAUCAUGUAGGCUUAAGCCGUAGCAC-3’
   - **Corresponding DNA Sequence:**
     - mRNA is synthesized from the template DNA strand, which runs in the 3' to 5' direction.
     - The coding DNA strand is complementary to the template strand and identical to the mRNA sequence (except T replaces U).

2. **Identify and Indicate 3’ and 5’ Ends on Both Strands:**
   - The template strand is complementary and antiparallel to the mRNA.
   - The coding strand is parallel and has the same sequence directionality as the mRNA, swapping uracil (U) for thymine (T).

3. **Determine Template and Coding Strands:**
   - **Template Strand:** Serves as the base for mRNA synthesis. It pairs with the mRNA using complementary base pairing rules.
   - **Coding Strand:** Has the same sequence as the mRNA (with T instead of U) and indicates the direction of coding.

### Analysis and Details

When transcribing DNA to mRNA:
- Adenine (A) pairs with Uracil (U)
- Uracil (U) pairs with Adenine (A)
- Cytosine (C) pairs with Guanine (G)
- Guanine (G) pairs with Cytosine (C)

#### Example Transcription

- **Template Strand (3’ to 5’):** 
  - 3’-CAGTCTATAGTACATCCGAATTCGGCATCGTG-5’

- **Coding Strand (5’ to 3’):** (matches mRNA sequence except T for U) 
  - 5’-GTCAGATATCATGTAGGCTTAAGCCGTAGCAC-3’

This transcription illustrates how mRNA is built using the template strand, while the coding strand reflects the nucleotide sequence of the mRNA.
Transcribed Image Text:**Using the mRNA Sequence Analysis** The task involves analyzing the given mRNA sequence: 5’-GUCAGAUAUCAUGUAGGCUUAAGCCGUAGCAC-3’ ### Objective To determine the original double-stranded DNA sequence used to synthesize the above mRNA sequence and indicate specifics regarding the DNA strands. ### Instructions 1. **Write out the Double-Stranded DNA Sequence:** - **mRNA Sequence:** 5’-GUCAGAUAUCAUGUAGGCUUAAGCCGUAGCAC-3’ - **Corresponding DNA Sequence:** - mRNA is synthesized from the template DNA strand, which runs in the 3' to 5' direction. - The coding DNA strand is complementary to the template strand and identical to the mRNA sequence (except T replaces U). 2. **Identify and Indicate 3’ and 5’ Ends on Both Strands:** - The template strand is complementary and antiparallel to the mRNA. - The coding strand is parallel and has the same sequence directionality as the mRNA, swapping uracil (U) for thymine (T). 3. **Determine Template and Coding Strands:** - **Template Strand:** Serves as the base for mRNA synthesis. It pairs with the mRNA using complementary base pairing rules. - **Coding Strand:** Has the same sequence as the mRNA (with T instead of U) and indicates the direction of coding. ### Analysis and Details When transcribing DNA to mRNA: - Adenine (A) pairs with Uracil (U) - Uracil (U) pairs with Adenine (A) - Cytosine (C) pairs with Guanine (G) - Guanine (G) pairs with Cytosine (C) #### Example Transcription - **Template Strand (3’ to 5’):** - 3’-CAGTCTATAGTACATCCGAATTCGGCATCGTG-5’ - **Coding Strand (5’ to 3’):** (matches mRNA sequence except T for U) - 5’-GTCAGATATCATGTAGGCTTAAGCCGTAGCAC-3’ This transcription illustrates how mRNA is built using the template strand, while the coding strand reflects the nucleotide sequence of the mRNA.
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