RNA interference is a mechanism of gene silencing that is mediated by the presence of double stranded RNA. Arrange the steps involved in gene silencing by RNAi.

Q: Northern blots are valuable tools to analyze the mRNA level present in a sample. Describe an…

A: The goal of a northern blot is to monitor the expression of a particular genome in tissues, organs,…

Q: Draw out an example of a plasmid operon set up using amp resistance (outside the arabinose operon),…

A: Plasmid is the extrachromosomal DNA generally found in bacteria (prokaryotic cell) that can…

Q: Which one of the following amino acids is phosphorylated during the process of promoter clearance by…

A: In eukaryotes RNA Polymerase II is responsible for the synthesis of mRNA. The largest subunit of Pol…

Q: 1,What are the two main purposes of mitosis? 2,Why would we expect larger animals with longer…

A: Mitosis is a process of cell division that results in two genetically identical daughter cells…

Q: Noncoding ribonucleic acid (RNA) interference/regulation in reguards to gene expression.  Provide…

A: A human genome consists of both coding and noncoding RNAs and the population of noncoding RNAs is…

Q: Consider the Rho-dependent terminator sequence 5’CCCAGCCCGCCUAAUGAGCGGCCUUUUUUUU-3’. What affect…

A: The mutation is the change in the nucleotide sequence of the DNA. The point mutation is the mutation…

Q: Give the result(s) of the experiment in "MicroRNAs Control De Novo DNA Methylation Through…

A: INTRODUCTION MicroRNAs (miRNAs) are a class of small, single-stranded non-coding RNAs that regulate…

Q: During experimental RNAi, how does the researcher affect expression of a target gene? Group of…

A: Introduction: In the process of RNA interference(RNAi) double-stranded RNA molecule suppresses the…

Q: control medium that demonstrates the general growth rate of mutant candidates

A: Control Medium: This medium is used to demonstrate the general growth rate of mutant candidates in…

Q: RNA-induced gene silencing can be accomplished by cleaving and degrading mRNA encoding for a…

A: The control of gene expression is far more complex in eukaryotes than in prokaryotes. the gene…

Q: Discuss the two ways a bacterium can terminate transcription:  intrinsic and rho dependent.

A: Introduction :- Transcription is the process by which genetic information encoded in DNA is used to…

Q: Northern blotting depends on the phenomenon of the binding of aprobe to mRNA. In this technique,…

A: Northern Blotting is a blotting technique used to detect the presence of a specific ribonucleic acid…

Q: What is the regulatory region of the Trp operon that is involved in alternative transcription…

A: A set of genes known as the trp operon is discovered in the bacteria E. coli and codes for the…

Q: What is the concept behind RNAI? Explain in terms of how it works.

A: A "nucleic acid" is a linear polymer of nucleotides that is a component of the cell's information…

Q: Consider a gene being transcribed at a constant rate k1 and being degraded with first  order…

A: The genetic information from protein synthesis is stored in DNA. This information is copied onto an…

Q: Describe how RNA primers are removed in eukaryotes.

A: DNA replication is a process in which two DNA molecules are synthesized from a single DNA molecule.…

Q: A particular mutation in the bacterial sigma factor allows this protein to bind RNA polymerase but…

A: Answer - Option A - It will prevent the transcription termination exerted by the Rho protein

Q: How to incorporate prokaryotic gene into eukaryotic cell to make an transgenic organism?In this case…

A: Transformation is a technique used to incorporate foreign DNA into a target organism. In this case,…

Q: Why is it easy for researchers to know what gene is targeted in an RNA interference experiment but…

A: RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression or…

Q: High levels of glucose in the environment result in low levels of transcription of the lac operon.…

A: Answer- glucose lowers the levels of cAMP, which is necessary to activate the CAP protein

Q: Suppose that E. coli sustains a mutation in its gene for the lac operon repressor making the…

A: When lactose is not present in the medium operon does not transcribe because of the presence of lac…

Q: RNA splicing

A: Most eukaryotic cells undergo post-transcriptional modification, which involves chemically altering…

Q: In bacteria, genes that are often used together are controlled by a single promoter. Explain why…

A: Gene regulation refers to the numerous processes that regulate which genes are expressed and the…

Q: For what purpose the process of RNA interference has been used?

A: Ribonucleic acid (RNA) are polymeric organic molecules, which are essential for carrying out various…

Genomics

Genes are responsible for transmitting the characters located on the chromosomes from one individual to another across the generations. Genetics is the study of different genes that are involved in different functions. Genetics is a branch of biology that studies genes, genetic diversity, and heredity in living organisms. The genetic system consists of genetic notations, which is a system of signs and symbols. Genotype refers to the organism's genetic composition, and phenotype refers to the observable property of the organism.