Question 7: Peptide nucleic acids (PNAS) exhibit greater stability as heteroduplexes with DNA (ie PNA-DNA duplex) than does double-stranded DNA (i.e. DNA-DNA duplex). However, peptide nucleic acids lack charged groups, making them largely insoluble under near-physiological conditions in aqueous buffer. Provide (1) an explanation for the increased stability of PNA-DNA duplexes (hint: consider intermolecular forces). (2) Additionally, propose modification(s) of the PNA scaffold DNA HO. that could increase solubility without drastically reducing duplex stability. PNA R1 HN Base Base Base Base =P-O° NH Base Base Но NH R2
Question 7: Peptide nucleic acids (PNAS) exhibit greater stability as heteroduplexes with DNA (ie PNA-DNA duplex) than does double-stranded DNA (i.e. DNA-DNA duplex). However, peptide nucleic acids lack charged groups, making them largely insoluble under near-physiological conditions in aqueous buffer. Provide (1) an explanation for the increased stability of PNA-DNA duplexes (hint: consider intermolecular forces). (2) Additionally, propose modification(s) of the PNA scaffold DNA HO. that could increase solubility without drastically reducing duplex stability. PNA R1 HN Base Base Base Base =P-O° NH Base Base Но NH R2
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
![Question 7:
Peptide nucleic acids (PNAS) exhibit greater stability as heteroduplexes with DNA (ie PNA-DNA duplex) than does
double-stranded DNA (i.e. DNA-DNA duplex). However, peptide nucleic acids lack charged groups, making them largely
insoluble under near-physiological conditions in aqueous buffer. Provide (1) an explanation for the increased stability of
PNA-DNA duplexes (hint: consider intermolecular forces). (2) Additionally, propose modification(s) of the PNA scaffold
DNA
that could increase solubility without drastically reducing duplex
stability.
PNA
R1
HN
но.
Base
Base
o=P-O
NH
Base
Base
8
o=P-O-
NH
8.
Base
Base
НО
NH](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fa882ecd1-7300-4dff-bf30-691af686de59%2Fb96d4163-f692-4a28-b728-5eb778aef7b5%2Ffz2jwii_processed.png&w=3840&q=75)
Transcribed Image Text:Question 7:
Peptide nucleic acids (PNAS) exhibit greater stability as heteroduplexes with DNA (ie PNA-DNA duplex) than does
double-stranded DNA (i.e. DNA-DNA duplex). However, peptide nucleic acids lack charged groups, making them largely
insoluble under near-physiological conditions in aqueous buffer. Provide (1) an explanation for the increased stability of
PNA-DNA duplexes (hint: consider intermolecular forces). (2) Additionally, propose modification(s) of the PNA scaffold
DNA
that could increase solubility without drastically reducing duplex
stability.
PNA
R1
HN
но.
Base
Base
o=P-O
NH
Base
Base
8
o=P-O-
NH
8.
Base
Base
НО
NH
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