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QUESTION 6 COO 21 CH₂ 31 CH-COO 21 HO-C-H 31 COO- Isocitrate AG= -8.4kJ/mol AG'= -17kJ/mol NAD NADH Oe. NADH solution f. NAD solution g. Isocitrate dehydrogenase mixture Oh. Isocitrate solution +H CO₂ COO 1 CH₂ 31 CH, CHO COO a-ketoglutarate You want to measure the activity of isocitrate dehydrogenase from a tissue sample by following the production of NADH. Which of the following components would you need to include in your assay mixture? a. Water Ob. Bicarbonate solution Oc. Phosphate buffer solution (pH7.5) d. a-ketoglutarate solution

QUESTION 6 COO 21 CH₂ 31 CH-COO 21 HO-C-H 31 COO- Isocitrate AG= -8.4kJ/mol AG'= -17kJ/mol NAD NADH Oe. NADH solution f. NAD solution g. Isocitrate dehydrogenase mixture Oh. Isocitrate solution +H CO₂ COO 1 CH₂ 31 CH, CHO COO a-ketoglutarate You want to measure the activity of isocitrate dehydrogenase from a tissue sample by following the production of NADH. Which of the following components would you need to include in your assay mixture? a. Water Ob. Bicarbonate solution Oc. Phosphate buffer solution (pH7.5) d. a-ketoglutarate solution

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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est on the Prac X pps Bb Reassessments X Bb About this Modi X * Question Completion Status: QUESTION 6 Secure https://blackboard.le.ac.uk/webapps/assessment/take/launch.jsp?course_assessment_id=_39582_1&course_id=_41417_1&content_id= Iv Bag, Medical Palmar Oceanfron... Glv Bag, Medical Dr... Amazing Opportu... Unavailable in you... COO CH₂ CH-COO- 21 HO-C-H S COO- Isocitrate 4 OL AG = -8.4kJ/mol AG' = -17kJ/mol NAD Bb About this Modi X Bb Practical report X esc NADH 2 + H+ You want to measure the activity of isocitrate dehydrogenase from a tissue sample by following the production of NADH. Which of the following components would you need to include in your assay mixture? a. Water b. Bicarbonate solution c. Phosphate buffer solution (pH7.5) d. a-ketoglutarate solution e. NADH solution f. NAD solution g. Isocitrate dehydrogenase mixture Oh. Isocitrate solution F2 CO Click Save and Submit to save and submit. Click Save All Answers to save all answers. QUESTION 7 0.25mL of a stock solution of ortho-nitrophenol (ONP) is diluted to a final volume of 3mL in a cuvette. You measure the absorbance at 410nm of the ONP in the cuvette. This gives a reading of 0.723 absorbance units Given that the molar absorption coefficient, e, for ONP is 3.47 x 103 M-1 cm-1 and the path length is 1cm, what is the concentration of the ONP in the stock solution? Oa. 2.08x104 mM 20 # 2C007 CH₂ 3 CH₂ C=0 000 a-ketoglutarate 3 G F3 $ R Bb Take Test: Prac X % 5 Dr... JD Fila Disruptor II R... 3. Edexcel Revisio... p Edexcel MacBook Pro F5 T Answered: Lisa X F6 57 Y * CO 8 U Bioch F8 a A
Transcribed Image Text:est on the Prac X pps Bb Reassessments X Bb About this Modi X * Question Completion Status: QUESTION 6 Secure https://blackboard.le.ac.uk/webapps/assessment/take/launch.jsp?course_assessment_id=_39582_1&course_id=_41417_1&content_id= Iv Bag, Medical Palmar Oceanfron... Glv Bag, Medical Dr... Amazing Opportu... Unavailable in you... COO CH₂ CH-COO- 21 HO-C-H S COO- Isocitrate 4 OL AG = -8.4kJ/mol AG' = -17kJ/mol NAD Bb About this Modi X Bb Practical report X esc NADH 2 + H+ You want to measure the activity of isocitrate dehydrogenase from a tissue sample by following the production of NADH. Which of the following components would you need to include in your assay mixture? a. Water b. Bicarbonate solution c. Phosphate buffer solution (pH7.5) d. a-ketoglutarate solution e. NADH solution f. NAD solution g. Isocitrate dehydrogenase mixture Oh. Isocitrate solution F2 CO Click Save and Submit to save and submit. Click Save All Answers to save all answers. QUESTION 7 0.25mL of a stock solution of ortho-nitrophenol (ONP) is diluted to a final volume of 3mL in a cuvette. You measure the absorbance at 410nm of the ONP in the cuvette. This gives a reading of 0.723 absorbance units Given that the molar absorption coefficient, e, for ONP is 3.47 x 103 M-1 cm-1 and the path length is 1cm, what is the concentration of the ONP in the stock solution? Oa. 2.08x104 mM 20 # 2C007 CH₂ 3 CH₂ C=0 000 a-ketoglutarate 3 G F3 $ R Bb Take Test: Prac X % 5 Dr... JD Fila Disruptor II R... 3. Edexcel Revisio... p Edexcel MacBook Pro F5 T Answered: Lisa X F6 57 Y * CO 8 U Bioch F8 a A
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