est on the Prac XppsBb Reassessments XBb About this Modi X* Question Completion Status:QUESTION 6Secure https://blackboard.le.ac.uk/webapps/assessment/take/launch.jsp?course_assessment_id=_39582_1&course_id=_41417_1&content_id=Iv Bag, MedicalPalmar Oceanfron... Glv Bag, Medical Dr...Amazing Opportu...Unavailable in you...COOCH₂CH-COO-21HO-C-HSCOO-Isocitrate4OLAG = -8.4kJ/molAG' = -17kJ/molNADBb About this Modi X Bb Practical report XescNADH2+ H+You want to measure the activity of isocitrate dehydrogenase from a tissue sample by following the production of NADH. Which of the following components would youneed to include in your assay mixture?a. Waterb. Bicarbonate solutionc. Phosphate buffer solution (pH7.5)d. a-ketoglutarate solutione. NADH solutionf. NAD solutiong. Isocitrate dehydrogenase mixtureOh. Isocitrate solutionF2COClick Save and Submit to save and submit. Click Save All Answers to save all answers.QUESTION 70.25mL of a stock solution of ortho-nitrophenol (ONP) is diluted to a final volume of 3mL in a cuvette. You measure the absorbance at 410nm of the ONP in the cuvette.This gives a reading of 0.723 absorbance unitsGiven that the molar absorption coefficient, e, for ONP is 3.47 x 103 M-1 cm-1 and the path length is 1cm, what is the concentration of the ONP in the stock solution?Oa. 2.08x104 mM20#2C007CH₂3CH₂C=0000a-ketoglutarate3GF3$RBb Take Test: Prac X%5Dr... JD Fila Disruptor II R... 3. Edexcel Revisio... p EdexcelMacBook ProF5TAnswered: Lisa XF657Y* CO8UBiochF8aA

Isocitrate dehydrogenase is an enzyme involved in the TCA cycle. The TCA cycle occurs in the…

Answered: QUESTION 6 COO 21 CH₂ 31 CH-COO 21…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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QUESTION 27 Which of the following is INCORRECT about the elimination of NH4 O a. Marine invertebrate excrete NHT in the form of urea O b. All amino groups are collected in glutamate OC NH, is a very toxic substance O d. Birds remove excess ammonium ions as uric acid QUESTION 28 All of the following are CORRECT regarding arginase EXCEPT: O a. Catalyzes the hydrolysis of arginine Ob. Present only in vertebrates Oc Present in the mitochondrial matrix O d. Regenerate ornithine
Question 7 Identify the functional groups in the following structures. HO 1. 12345 1. HO- 3. GAWNE Answers 15 1. 2. FLAG QUESTION adrenaline QUESTIONS VERSION 3M 44 CORS NH 3. 4. ОН 2. NH₂ amphetamine
QUESTION 19 Which enzyme (Enzyme 1, circle; Enzyme 2 square) has the worst affinity for the substrate? 5.0 4.0 EnzGraph 3.0 8 2.0 1.0 0.0 10 15 20 25 30 35 40 45 50 substrate (mM) Enz1 Enz2 A. Enzyme 1 (circles) B. Enzyme 2 (squares) C. cannot tell with information given D. both have the same affinity
Question 11 The breakdown of large molecules by the enzymatic addition of water is an example of what kind of reaction? O 1. oxidation O 2. reduction O 3. condensation O 4. hydrolysis O 5. decarboxylation A Moving to another question will save this response. W AR SONY ASSIST BUtton, Onetouch
QUESTION 18 Which of the following reactions is energetically favorable? The formation of peptide bonds O The addition of a phosphate.group to ADP O The breaking of glycosidic bonds O The formation of phosphodiester bonds
Question 28 Which enzyme breaks glucose into 2 molecules of 3 carbons each? O pyruvate deydrogenase O phosphoglycerokinase Aldolase O phosphoglucoisomerase O Hexokinase
Question #4
Question 1: In some microorganisms, carbon fixation occurs by reversal of the citric acid cycle. This reversal is accomplished in part by the use of a strong reductant (ferredoxin) to drive the alpha-ketoglutarate dehydrogenase reaction in the reductive direction. Part a: ΔG°‘ for reaction as it occurs in the ‘normal’ (oxidative) citric acid cycle is -30.1 kJ/mol. The standard reduction potential for NADH is -0.32 V. In order to drive the reaction in the reverse direction, the reductant (a ferredoxin) must have a lower reduction potential than NADH/NAD+. Remembering that this is a two-electron reduction, and using the numbers given just above, compute the value of the ferredoxin reduction potential that would be needed to make the standard free energy zero (so that the reductive reaction is enegetically just as favorable as the oxidative reaction). Assume that all of the other reactants are the same in the reductive as in the oxidative reaction. Write out the steps in your calculation;…
QUESTION 37 Key enzymes in glycolysis and the Krebs cycle are allosterically regulated. Which of the following is FALSE? O a. The main control point of glycolysis is the enzyme phosphofructokinase. Ob. The main control point in the citric acid cycle is isocitrate dehydrogenase Oc. Isocitrate dehydrogenase is inhibited by ATP or NADH. O d. Phosphofructokinase is activated by citrate and ATP. Oe. If ATP levels are high and the citric acid cycle shuts down, acetyl CoA is diverted to the synthesis of fatty acids for storage lick Save and Submit to save and submit. Click Save All Answers to save all answers. S # 20 F3 $ F4 % L5 MacBook Pro
Question 9 Match the enzyme with a phrase about it. v Catalyzes a substrate lovei phosphorylation 3. Pyruvate kinaise v breaks down sucrose to glucose and fructose b. phosphovylase e phosphohaxesa semerase (ato called giasose-6 phosphete samerane) v is a type of isomerase v removes one glucose residue from glycogen d. Sucrase A Muving to another question will save this response
Question 6 2. In premature infants, the synthesis of lung surfactants (including phospholipids) is decreased, partly contributing to impaired lung functions. Explain how pulmonary surface tension and lung compliance will change with decreased lung surfactants in premature infants (mention if pulmonary surface tension and lung compliance each will increase or decrease with decreased lung surfactants, and why).
QUESTION 20 Which enzyme (Enzyme 1, circle; Enzyme 2 square) has the best affinity for the substrate? 5.0 4.0 3.0 EnzGraph 8 2.0 1.0 0.0 10 15 20 25 30 35 40 45 50 substrate (mM) Enz1 Enz2 A. both have the same affinity B. Enzyme 2 (squares) C. cannot tell with information given D. Enzyme 1 (circles)

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