QUESTION 4Suppose you have a length of DNA sequence that corresponds to a complete mRNA open reading frame(coding region) for a protein enzyme (i.e. the region from the translation start codon reading continuouslythrough to the translation stop codon). Which of the following might be a way to help determine if the DNA isfrom human or E. coli?O A.Examine the translation start codon, it should be only be AUG (ATG) if the DNA is from E. coli.O B. Digest with a restriction enzyme, bacterial DNA should be cut but human DNA should not.O C. Compare the codon usage to E. coli and human codon usage tables.O D. Look for splice sites GT-AG, those should only be present if the DNA is from human.O E. Both B and D

Name: QUESTION 4Suppose you have a length of DNA sequence that corresponds
Uploaded: 2024-03-20T06:42:41.000Z
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Description: QUESTION 4Suppose you have a length of DNA sequence that corresponds to a complete mRNA open reading frame(coding region) for a protein enzyme (i.e. the region from the translation start codon reading continuouslythrough to the translation stop codo

Q4. The correct option is D i.e, look for splice sites GT-AG thise should only be present if the DNA is from human. Explanation: This is because GT-AG splicing sites are mostly present in eukaryotes. Most bacterial RNA transcripts do not splice; instead, they are colinear, with DNA directly encoding them. Others are incorrect. a. In both humans and E. Coli both have AUG as a start codon, hence incorrect b. Restriction enzymes cleave both humans as well as e.coli DNA, hence incorrect Thus correct option is D.