QUESTION 283.0All of the following statements about the Illumina (reversible terminator) process for carrying out Next Generation Sequencing (NGS) are true EXCEPT:O A. The template is fragmented into small oligonucleotides with read lengths of 400-500 nucleotides.O B. The template fragments are attached to a glass slide where they are amplified by PCR.OC The different nucleotides (A,C,G,T) that are added during the sequencing reaction are identified byfluorescent labels of different colors.O D. In cases where the same nucleotide multiple times in adjacent positions, the number of nucleotide repeats is determined by the intensity of thefluorescent light flash.O E. The 3' carbon of the labeled nucleotides that are added during the sequencingreaction is blocked.

Next-Generation sequencing also called high throughput sequencing or second-generation sequencing or…

Answered: QUESTION 28 All of the following…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question 4
QUESTION 2 Which of the following would be classified as non-coding DNA? (mark all applicable answers) The cytochrome oxidase Il gene found on the human mitochondrion Introns of the alpha hemoglobin gene found on human chromosome 16 Exons of the alpha hemoglobin gene found on human chromosome 16 U The DNA between genes in the human genome (also referred to as intergenic regions or IGRS)
Question 47
Question 2. From mouse nerve cells you isolate the mouse Tau-gene-containing DNA fragment and hybridize it to the mRNA encoding Tau from the cytoplasm of the same cells. Describe what you see when you look at the molecules under the electron microscope. A. A double-stranded DNA-RNA hybrid with complete overlap of all the sequences. B. A partially double stranded DNA-RNA hybrid molecule containing regions of DNA-RNA overlap representing the mRNA exons, loops where there are introns and tails at the 5' and 3' ends. C. A partially double-stranded molecule containing regions of DNA-RNA overlap representing the mRNA introns, loops where there are introns and tails at the 5' and 3' ends. D. Regions of DNA-RNA overlap representing the mRNA exons and loops where there are introns with no tails at the 5' or 3' ends. E. Two distinct molecules with no overlapping regions. Multiple Choice
Question 48
Question 5 A culture of E. coli growing in a medium containing the normal, lighter isotope of nitrogen (¹4N) as the sole source of nitrogen is transferred to a medium containing the heavier isotope of nitrogen (15N) for 7 generations. What is the molar ratio (expressed as a decimal to the nearest thousandths) of the hybrid DNA (15N-14N) to heavy DNA (15N-15N) at this point? Hint: try to recognize the pattern represented by the above process to answer this question more efficiently.
Question 6 If the plasmid were completely digested with the two restriction enzymesBamHI andHgiEII, the resulting fragments would be this large: Clal (24) ... EcoRI (4359) Hindi (29) EcoRV (187) Apali (4033) BamHI (375) Pstl (3611) HaiEll (3056) 3400 3200 3000 Apall (2787) 2800 Sspl (4170) a-lactamase gene 3800 400 Afull A) 3056, 2295, 375 base pairs long B) 1980, 1620 and 761 base pairs long C) 1920, 1305, 761 and 375 base pairs long D 1920, 1680, 761 base pairs long 2600 pBR322 4361bp 2200 2400 Hai Ell (2295) 200 400 600 tetracycline resista 800 1800 1600 2000 rop Pvull (2066) 1000 Sphi (566) 004 1400 Sall (651)
QUESTION 3 Look at the following piece of DNA cut with enzymes I and II, crating 3 fragments, A, B and C Which of these is the correct configuration for how they would run on a gel? (remember that DNA is negatively charged and runs towards the positive electrode). II DNA: A C 1. O A C 2. O A C 3. O C A 0 1 3.
QUESTION 7 The recognition sites for the restriction enzymes BamHI, Xbal, and BglII are G^GATCC, T^CTAGA, and A^GATCT, respectively. Which enzymes leave compatible ends that can be ligated together? O A. All of these enzymes leave ends that are compatible with ends generated by the others. O B. None of the enzymes produce compatible ends. C. Only BamHI and BglII fragments are compatible. O D. Only BamHI and Xbal fragments are compatible. O E. Only BglII and Xbal fragments are compatible.
QUESTION 16 Which of the following best describes the proper sequential order of the dogma of molecular genetics? O A. Deoxyribonucleic acid is converted to a pre-messenger ribonucleic acid that is modified to a messenger ribonucleic acid that is converted to a polypeptide chain, which is modified to a protein. B. Nucleic acid is converted to a pre-messenger ribonucleic acid that is modified to a messenger ribonucleic acid, which t is converted to a polypeptide chain that is modified to a protein. OC. Nucleotide is converted to a messenger ribonucleic acid that is modified to a polypeptide chain, which is modified to a protein. O D. Polynucleotide is converted to a pre-messenger ribonucleic acid that is modified to a messenger ribonucleic acid that is converted to a polypeptide chain, which is modified to a protein. O E. None of the answers (A, B, C, and D) is the correct answer choice for this question. QUESTION 17 Which of the following statements is precisely correct/true? O A.…
QUESTION 6 A new chemical pesticide developed for agricultural use was subjected to the Ames test. The results are as follows: Sample Number of his* revertent colonies 1 distilled water distilled water + rat liver enzymes pesticide pesticide + rat liver enzymes 125 127 Which of the following statements could you make about this pesticide? O The pesticide is modified by liver enzymes. O The pesticide is unlikely to be a mutagen in itself. O The pesticide may be protective against spontaneous mutation. O The pesticide is a mutagen O 1 and 4 are correct

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