Question 12: Why is the first step of this procedure (the mixing of haploids) performed on rich plates?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question 12: Why is the first step of this procedure (the mixing of haploids) performed on rich
plates?
Because they are auxotrophs, they need the appropriate nutrients to grow and then to mate. The cells can't
mate efficiently if they are not able to undergo normal metabolism.
To ensure the cells have enough nutrition to grow on the other minimal plates.
We could have used any type of plate. We happened to choose the rich plates for this experiment.
To ensure no mutations occur.
We perform this procedure on rich plates in order to create a control for the experiments. If we mixed the
haploids on UMM plates, we would not have a prototrophic control strain for the experiment.
Transcribed Image Text:Question 12: Why is the first step of this procedure (the mixing of haploids) performed on rich plates? Because they are auxotrophs, they need the appropriate nutrients to grow and then to mate. The cells can't mate efficiently if they are not able to undergo normal metabolism. To ensure the cells have enough nutrition to grow on the other minimal plates. We could have used any type of plate. We happened to choose the rich plates for this experiment. To ensure no mutations occur. We perform this procedure on rich plates in order to create a control for the experiments. If we mixed the haploids on UMM plates, we would not have a prototrophic control strain for the experiment.
Using the data provided below and the information from the Activity 3's Experiment section, answer
the following questions: Questions 12-15.
Light tan represents the spots where the inoculum was plated, but showed no growth.
Dark Tan represents the spots where inoculum was plated, but did show growth.
UMM
Transcribed Image Text:Using the data provided below and the information from the Activity 3's Experiment section, answer the following questions: Questions 12-15. Light tan represents the spots where the inoculum was plated, but showed no growth. Dark Tan represents the spots where inoculum was plated, but did show growth. UMM
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