Question 1. How cell separation is key in cell culture establishment?
Question 2. What is the relationship of adherent cells and trypsin in Animal cell culture?
Question 3. An enriched medium containing salts, glucose, proteins and vitamins was made and a commercially available animal cell line was introduced. However, the cells began dying. What could be the reason behind it?
Question 4. A) What is the significance of producing monoclonal antibodies?
- B) What is the role of cell culture in production of monoclonal antibodies?
- C) Name and briefly explain the use of any 4 commercially available monoclonal
Question 5. The aim of a research project is to produce a hybrid plant having resistance to disease. The disease resistance trait is cytoplasmically encoded. The parent plants which are chosen for the development of the hybrid are sexually incompatible. Which tissue culture technique will be used to produce a hybrid using these sexually incompatible parents.
Question 6. Agraobacterium mediated transformation is a useful gene transfer technique but still we can’t use Ti/ Ri plasmid directly as a vector. Why?
Question 7. Selection is an important step in genetic engineering. You are given ampicillin and tetracycline antibiotics. Using these antibiotics, which selection technique could be used to differentiate between recombinant and non-recombinant cells?
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