QUESTION 1 As your first experiment as a graduate student, your advisor asks you to reconstitute purified bacteriorhodopsin, a light- driven H+ pump from the plasma membrane of photosynthetic bacteria, and purified ATP synthase from ox-heart mitochondria together in the same membrane vesicles, as shown on the right. You work out a method so that all of the bacteriorhodopsin pumps H+ in one direction, into the vesicles. You then add ADP and Pi to the external medium and shine light onto the suspension of vesicles. What do you observe? purified ATP synthase purified bacteriorhodopsin detergent ADD PHOSPHOLIPIDS AND REMOVE DETERGENT LIGHT sealed vesicle (liposome) ete Garland Science 2010) A No ATP synthesis since no electron transport occurs. Figure 014-18 Esential Cell Biolegy le e B. ATP synthase makes ATP. CATP synthase hydrolyzes ATP to pump H+ into the vesicles. D.No ATP synthesis since no H+ gradient is created.
QUESTION 1 As your first experiment as a graduate student, your advisor asks you to reconstitute purified bacteriorhodopsin, a light- driven H+ pump from the plasma membrane of photosynthetic bacteria, and purified ATP synthase from ox-heart mitochondria together in the same membrane vesicles, as shown on the right. You work out a method so that all of the bacteriorhodopsin pumps H+ in one direction, into the vesicles. You then add ADP and Pi to the external medium and shine light onto the suspension of vesicles. What do you observe? purified ATP synthase purified bacteriorhodopsin detergent ADD PHOSPHOLIPIDS AND REMOVE DETERGENT LIGHT sealed vesicle (liposome) ete Garland Science 2010) A No ATP synthesis since no electron transport occurs. Figure 014-18 Esential Cell Biolegy le e B. ATP synthase makes ATP. CATP synthase hydrolyzes ATP to pump H+ into the vesicles. D.No ATP synthesis since no H+ gradient is created.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:## Question 1
As your first experiment as a graduate student, your advisor asks you to reconstitute purified bacteriorhodopsin, a light-driven H\(^+\) pump from the plasma membrane of photosynthetic bacteria, and purified ATP synthase from ox-heart mitochondria together in the same membrane vesicles, as shown on the right. You work out a method so that all of the bacteriorhodopsin pumps H\(^+\) in one direction, into the vesicles. You then add ADP and Pi to the external medium and shine light onto the suspension of vesicles. What do you observe?
### Diagram Explanation
The diagram depicts three main stages:
1. **Purification and Mixing**:
- **Purified Bacteriorhodopsin** and **Purified ATP Synthase** are mixed with detergent. This is represented by having the two proteins added together.
2. **Formation of Vesicles**:
- Phospholipids are added, and the detergent is removed, forming a sealed vesicle (liposome) with the bacteriorhodopsin and the ATP synthase embedded in the membrane.
3. **Light Activation**:
- Upon shining light, bacteriorhodopsin pumps H\(^+\) ions into the vesicle, creating a proton gradient. This is shown as light hitting the vesicle and H\(^+\) ions moving one way.
### Possible Observations
- **A.** No ATP synthesis since no electron transport occurs.
- **B.** ATP synthase makes ATP.
- **C.** ATP synthase hydrolyzes ATP to pump H\(^+\) into the vesicles.
- **D.** No ATP synthesis since no H\(^+\) gradient is created.
**Figure Reference**: Figure 014-18, Essential Cell Biology 3e (© Garland Science 2010)
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