Q 2: Solve the followings (a) The name "complex media" means that we do not know what is in it, but it is a poor media  True  False (b) Darkfield microscopy is a better choice to observe transparent organisms compared to conventional brightfield microscopy.   True  False  (c) Acid-fast staining is sued to differentiate bacteria that have a rich lipid content in their cell wall, such as the waxy mycolic acid. These complex lipid structures prevent staining of these cells using common stains and staining procedures. In Acid-fast staining, these cell walls are first made permeable to the stain by heating. The following decolorization using acid-alcohol solution will not remove the first dye from acid-fast cells.  True  False (d) Acid-fast staining is an important diagnostic tool  in the identification of Mycobacterium tuberculasis  True  False

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Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.