pyruvate] mM Reaction rate without compound X 10-9moles per second Reaction rate with compound X (10-9moles per second) 1 2.04 1.18 2 2.86 2.0 4 3.70 2.8 8 4.35 3.6 12 4.76 4.0 1(a). Estimate Vmax and Km for LDH with and without the inhibitor. 1(b). Determine the type of inhibition of the enzymatic reaction from the data the
The students were given a compound X. They are told that it inhibits LDH activity. The students
decided to check that by including it in the assay and testing the effect of the inhibitor using various
concentrations of substrate (pyruvate), in the presence of NADH. They evaluated the rates of the
LDH reaction with and without the inhibitor compound. Data obtained are shown below.
|
[pyruvate] mM |
10-9moles per second |
Reaction rate with compound X (10-9moles per second) |
|
1 |
2.04 |
1.18 |
|
2 |
2.86 |
2.0 |
|
4 |
3.70 |
2.8 |
|
8 |
4.35 |
3.6 |
|
12 |
4.76 |
4.0 |
1(a). Estimate Vmax and Km for LDH with and without the inhibitor.
1(b). Determine the type of inhibition of the enzymatic reaction from the data the students collected in the presence and absence of the inhibitor X.
1(c). Students were told that compound X is N-propyl oxamate. The structures of pyruvate and N-propyl oxamate are shown below. Is the information shown below consistent with your answer in question 1 part (b) ? Explain your answer.
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