Given this:ProteinIsoelectric pH Molecular weight (kDa)Ovalbumin4.645Insulin5.45.7Fibrinogen5.8340Y-globulin6.6160Collagen6.6115Hemoglobin (monomer)7.116Myoglobin7.016.7What is the appearance after visualization of a protein mixture containing the seven proteinsabove if isoelectric focusing is done?

The isoelectric point of a Protein: Isoelectric point is the pH at which a protein forms its…

Answered: Protein Isoelectric pH Molecular weight…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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What is the isoelectric point of the following peptide molecule? Asp-His-Glu-Gly-Lys-Tyr-Leu-Phe-Arg-Ser-Cys Relevant pKa values are; 2.2, 3.8, 4.3, 6.0, 8.3, 9.8, 10.1, 10.5, 12.5 and 13.1 3.0 9.1 7.2 5.2 4.1 ONLY one correct answer
1.0.1 mL of a protein solution of concentration of 11 mg/mL was diluted to a total volume of 4.0 mL with water (i.e. 0.1 mL of the solution was added to 3.9 mL of water). 2 mL of this solution was then mixed with 18 mL of water. What is the concentration of the diluted protein solution? Space to show your workings:
ok-Personal Microsoft Edge com/maildeeplink 021 pdt Download Print Save to OneDrive 1. Draw the structure of the pentapeptide Gln-Trp-His-Glu-Tyr that would predominate in aqueous solution at pH3D10.3. (Relevant pka values are: 2.2 4.3, 6.0, 9.8, and 10.1) Acp is adiusted to
draw condensed structural formula for monosaturated omega-3 fatty acid with 14 carbon atoms. draw the condensed structural formula for the triacylglycerol made from 3 saturated fatty acids that are 20 carbons long
Are all the proteins separated properly using two-dimensional (2D) gel electrophoresis? Why or why not?
Please draw titration curves for peptide HFDTYA and HEKDGQ, and calculate the pl for each. Usethis information to explain why the peptide HFDTYA has low solubility and HEKDGQ has a highersolubility in bulk water at pH = 7.
Major functional groups present and expected wavenumbers of the peaks: Attach IR spectrum of the compound and list wavenumbers of signals/peaks observed in the spectrum: Discussion
4d
Explain what the meaning of these distances is (e.g., from where to where?)Crystal structure of human monocyte chemotactic protein-2
The twisted proteins store elastic potential energy as it twists or untwists from its equilibrium state. The energy function for this system is shown in the figure where the energy is given in multiples of kBT which is a unit of energy used by biochemists when working at the molecular level. (1.0 kBT = 4.11×10−21 J). Figure 6 shows a horizontal line at an energy level of 136 kBT , which gives a total angular range of 2.095 radians. If the energy level of the ATP-synthase stalk is 100 kBT instead, between what angles is the stalk confined? Explain your reasoning.

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