Primer design for SLIC cloning and the protocol
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
describe each step carefully and use your own words. Steps will be like following;
I) Primer design for SLIC cloning and the protocol
II) Transformation and sequence validation of the clones
III) Culture of HEK293T
IV) Transduction of HEK293T cells with plasmid
V) Western Blotting
VI) Immunfluorescence for Confocal Microscopy
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