Pls explain If the voltage were NOT increased at the start of the resolving gel run, the overall experiment time will ______, while the calculated electrophoretic mobilities will ______. a. increase, increase b. increase, decrease c. decrease, increase d. decrease, decrease
Pls explain
If the voltage were NOT increased at the start of the resolving gel run, the overall
experiment time will ______, while the calculated electrophoretic mobilities will
______.
a. increase, increase
b. increase, decrease
c. decrease, increase
d. decrease, decrease
Pick all that are TRUE regarding analysis of quaternary structures of proteins
using polyacrylamide electrophoresis:
I. The added β-mercaptoethanol disrupts S--S bonds bridging the
polypeptide chains causing the appearance of higher Rf bands
compared to the native protein run.
II. Heating up any protein before subjecting to SDS-PAGE will always
result in the formation of more than one band.
III. A good asymmetrical gel layout would be : (Lane 1) MW ladder, (2)
native protein, (3) protein + β-ME, (4) protein + HCL, (5) protein +
β-ME + HCl.
IV. Formation of a single band in the protein + β-ME + HCl run, whose
Rf is lower than the native run, could be indicative that the protein is
a homodimer.
A. I only
B. I and II
C. II and IIV
D. None is true.
Step by step
Solved in 3 steps
