Place the following steps of DNA replication in order (from left to right) from the beginning to the end of the process.ResetHelppriming of DNAsynthesis with RNAprimerremoval of primers byDNA polymerase Iexonucleaseelongation ofRNA primers by DNApolymerase IIIopening andstabilizing the DNA strandsjoining of Okazakifragments by DNA ligaseBeginning of replicationEnd of replication

Name: Place the following steps of DNA replication in order (from left to r
Uploaded: 2024-03-20T06:42:41.000Z
Channel: Bartleby
Description: Place the following steps of DNA replication in order (from left to right) from the beginning to the end of the process.ResetHelppriming of DNAsynthesis with RNAprimerremoval of primers byDNA polymerase Iexonucleaseelongation ofRNA primers by DNApol

DNA (deoxyribonucleic acid) was discovered by Friedrich Miescher. Nucleotides are the structural units of DNA. A combination of a sugar molecule, phosphate group, and nitrogen bases is called a nucleotide. The DNA is made up of pentose sugar called deoxyribose, a phosphate group derived from phosphoric acid and nitrogen bases which are adenine, thymine, cytosine, and guanine. DNA is the genetic material in most organisms except few viruses. The genetic material has to be chemically stable with the capacity of replication with minimum errors. The structure of DNA was proposed by James Watson and Francis Crick based on X-ray crystallography.The process in which the DNA molecule prepares an exact copy of itself is called replication. The semiconservative model of replication was given by Meselson-Stahl after performing an experiment on Escherichia coli. The semiconservative model states that as the DNA is unwound the two strands will be separated with each acting as a template for the complementary base pairing of the new strand. Thus after replication, both the DNA molecules will have one parental strand and one new strand, Various enzymes are needed in the process of DNA replication. The correct order fo the replication is Opening and stabilizing the DNA strands- The two DNA strands need to be separated for replication which is done by the DNA unwinding protein called DNA helicase. They are stabilized by single-stranded binding proteins. Priming of DNA synthesis by RNA primers- The RNA primer is synthesized by RNA primase and it initiates DNA replication. Elongation of the RNA primer by DNA polymerase III- DNA polymer III is responsible for the polymerization of the DNA. They add nucleotides on the template DNA strand in the 5' to 3' direction. It elongates the RNA primer on template DNA. Joining of the Okazaki fragments by DNA ligase- The one strand is synthesized continuously while there is a discontinuous synthesis on the other strand. This leads to the production of Okazaki fragments which are joined by DNA ligase. Removal of the primers by DNA polymerase I exonuclease- The primers are removed by DNA polymerase I exonuclease activity.