Ouabain specifically inhibits the Na+K+ ATPase activity of animal tissues but is not known to inhibit any other enzyme. When ouabain is added to thin slices of living kidney tissue, it inhibits oxygen consumption by 66%. Why? What does this observation tell us about the use of respiratory energy bykidney tissue?

Ouabain specifically inhibits the Na+K+ ATPase activity of animal tissues but is not known to inhibit any other enzyme. When ouabain is added to thin slices of living kidney tissue, it inhibits oxygen consumption by 66%. Why? What does this observation tell us about the use of respiratory energy bykidney tissue?

BIOLOGY:CONCEPTS+APPL.(LOOSELEAF)

10th Edition

ISBN:9781305967359

Author:STARR

Publisher:STARR

Chapter35: Respiration

Section: Chapter Questions

Problem 1CT

See similar textbooks

Related questions

Q: (a) NAD+ kinase catalyzes the ATP-dependent conversion of NAD* to NADP+. How many reducing…

A: The Nicotinamide Adenine Dinucleotide (NAD) acts as a coenzyme while, Nicotinamide Adenine…

Q: You are growing human muscles cells in the lab and you are trying to test how well the cells survive…

A: The muscles with alternate light and dark bands which remain associated with skeleton and can…

Q: Cells maintain different steady state ratios of NAD+/NADH (1000) and NADP+/NADPH (0.01). (a) How do…

A: NAD+ is the oxidized version. NAD+ accepts two electrons and a proton to get reduced to NADH.NAD+ +…

Q: In mammals of all species, the peak rate of O2 consumption of each mitochondrion is roughly the…

A: Mitochondria powerhouse of a cell because it provides energy for the functioning of a cell. It…

Q: an intermediate in glycolysis. Glycerol backbone of a fat molecule can be converted to…

A: Oxidative phosphorylation is a process of formation of ATP from oxidation of fat, carbohydrates and…

Q: What effect would cyanide have on NADH and NAD+ concentrations in a cell? In your response, you must…

A: Cyanide is a poison that inhibits the cellular respiration by binding to ferric ions of cytochrome…

Q: Unlike a rabbit, running all-out for a few moments to escape a predator, migratory birds require…

A: Acetyl coenzyme A is a key metabolic intermediate that is produced in a variety of methods,…

Q: What are differences between aerobic and anaerobic respiration? Name some organisms that use…

A: Cellular respiration is one of the metabolic reactions and mechanisms that occur in the cells of…

Q: After infection, some T cells (a particular type of immune cell) may persist as memory T cells…

A: Electron transport occur in the mitochondrial membrane and thus are used for the production of…

Q: (a) NAD+ kinase catalyzes the ATP-dependent conversion of NAD+ to NADP+. How many reducing…

A: NAD+ kinase is an enzyme that converts nicotinamide adenine dinucleotide into NADP+ through…

Q: 23 Samples of five plant tissue discs were incubated in dilute sodium chloride solution at different…

A: There are few important points to know : The success of tissue culture depends on the composition of…

Q: During aerobic respiration, in the mitochondria of animal cells, when diatomic oxygen molecules (O2)…

A: Aerobic respiration is a complex process occurring in four stages the first stage been glycolysis,…

Question

Ouabain specifically inhibits the Na⁺K⁺ATPase activity of animal tissues but is not known to inhibit any other enzyme. When ouabain is added to thin slices of living kidney tissue, it inhibits oxygen consumption by 66%. Why? What does this observation tell us about the use of respiratory energy by
kidney tissue?

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

What is the difference between respiration and aerobic cellular respiration?
Jogging is considered aerobic exercise because the cardiovascular system (heart and blood vessels) can adjust to supply the oxygen needs of working cells. In contrast, sprinting the 100-meter dash might be called anaerobic (lacking oxygen) exercise, and golf nonaerobic exercise. Explain these last two observations.
Figure 4.15 Cyanide inhibits cytochrome c oxidase, a component of the electron transport chain. If cyanide poisoning occurs, would you expect the pH of the intermembrane space to increase or decrease? What affect would cyanide have on ATP synthesis? Figure 4.15 (a) The electron transport chain is a set of molecules that supports a series of oxidation-reduction reactions. (b) ATP synthase is a complex, molecular machine that uses an H+ gradient to regenerate ATP from ADP. (c) Chemiosmosis relies on the potential energy provided by the H+ gradient across the membrane.
Which of the following is not produced by an animal muscle cell operating under anaerobic conditions? a. heat d. ATP b. pyruvate e. lactate c. PGAL f. oxygen
The rate at which the Na+K+ pump operates is not constant but is controlled by a combined effect of changes in ICF Na+ concentration and ECF K+ concentration. Do you think the changes in both ICF Na+ and ECF K+ concentration following a series of action potentials in a neuron would accelerate, slow down, or have no effect on the Na+K+ pumps in this cell?
As CO2 concentrations increase in the atmosphere, biologists continue to explore the role of respiration from plants as a small but potentially important contribution beyond fossil fuel combustion. The data in the Table were collected from the leaf of a sagebrush plant from a semiarid ecosystem in Wyoming, enclosed in a chamber that measures the rate of CO2 exchange. The respiration rate is the amount of CO2 in micromoles lost by the leaf per square meter per second, which results in the negative numbers. The temperature values are from the leaves as they are heated or cooled during the measurements. Observation Temperature (C) Respiration Rate (mol/m2/s) 1 25 -2.0 2 30 -2.7 3 35 -4.1 4 40 -5.8 5 20 -1.3 6 15 -1.0 7 10 -0.7 Cengage Learning 2017 What describes the relationship between temperature and respiration, a line or a curve? Does the Q10 that you calculated in 2 suggest a line or a curve? Source: Data based on unpublished research by Brent Ewers, University of Wyoming.
Using the Nernst equation, calculate the equilibrium potential for Ca2 and for C1 from the following sets of data: a. Given [ Ca2+ ]0=1mM,[ Ca2+ ]i=100nM, find Eca2+ b. Given [ Cl- ]0=110mM,[ Cl- ]i=100mM, find Ecl
Characterizing Glycolysis List the reactions of glycolysis that a. are energy consuming (under standard-stale conditions), b. are energy yielding (under standard-state conditions), c. consume ATP. d. yield ATP e. are strongly influenced by changes in concentration of substrate and product because of their molecularity. f. are at or near equilibrium in the erythrocyte (see Table 18.2).
13. There are several ways to measure cellular respiration experimentally. For example, CO2 and O2 gas sensors measure changes over time in the concentration of carbon dioxide or oxygen, respectively. Design two experiments to test the effects of changing two different variables or conditions (one per experiment) on the respiration of research organism of your choice.
Using the answer code on the right, indicate which form of energy production is being described: 1. takes place in the mitochondrial matrix 2. produces H2O as a by-product 3. results in a rich yield of ATP 4. takes place in the cytosol 5. processes acetyl-CoA 6. takes place in the mitochondrial innermembrane cristae 7. converts glucose into two pyruvate molecules 8. uses molecular oxygen 9. accomplished by the electron transport system and ATP synthase (a) glycolysis (b) citric acid cycle (c) oxidative phosphorylation
Figure 7.11 Dinitrophenol (DNP) is an "uncoupler" that makes the inner mitochondrial membrane "leaky" to protons. It was used until 1938 as a weight-loss drug. What effect would you expect DNP to have on the change in pH across the inner mitochondrial membrane? Why do you think this might be an effective weight-loss drug?
Test Your Understanding 13. INTEREPT DATA Look at Figure 46-10b. How would strenuous muscle activity affect the oxygenhemoglobin dissociation curve? (Hint: How does muscular activity affect pH? See Chapter 40 if you need to review this concept.) Figure 46-10 Oxygen-hemoglobin dissociation curves