ou are a bacteriologist studying a pathogenic protein (the “BAD” protein) that contributes to diseases caused by Staphylococcus aureus. BAD functions as an ab heterodimer, and the a subunit (25kD) and b subunit (75kD) are held together by an electrostatic interaction between K in the a subunit and D in the b subunit. You are trying to dissociate the BAD subunits to prevent pathogenesis in the bacteria. Which mutation would you make to prevent the BAD subunits from forming a dimer? Assume neutral pH.
You are a bacteriologist studying a pathogenic protein (the “BAD” protein) that contributes to diseases caused by Staphylococcus aureus. BAD functions as an ab heterodimer, and the a subunit (25kD) and b subunit (75kD) are held together by an electrostatic interaction between K in the a subunit and D in the b subunit.
You are trying to dissociate the BAD subunits to prevent pathogenesis in the bacteria. Which mutation would you make to prevent the BAD subunits from forming a dimer? Assume neutral pH.
(A) introduce AAA to AGA point mutation in the a subunit gene
(B) introduce GAC to GAG point mutation in the b subunit gene
(C) introduce GAT to CGT point mutation in the b subunit gene
(D) introduce two point mutations: AAG to GTG in the a subunit gene & GAC to CTC in the b subunit gene
(E) introduce two point mutations: AAA to TGC in the a subunit gene & GAT to TGT in the b subunit gene
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