One of the useful rules of thumb that you may have learned in Biochemistry, is that a 50 :g/ml solution of DNA has an absorbance of about 1.0 in a 1 cm cell. a. I have a solution of DNA that has an transmittance of 0.15 when it is placed in a cll with a 200 um pathlength. What is the concentration of DNA in this solution (in units of g/ml)? b. Assume that this DNA is a synthetic oligo containing 25 base pairs, and that the MW of a single base pair has a molecular weight of 330. What is the molar concentration of duplex DNA molecules in the solution? c. What is the Molar absorptivity of DNA in units of L cm (mol base pair) -1 -1
One of the useful rules of thumb that you may have learned in Biochemistry, is that a 50 :g/ml solution of DNA has an absorbance of about 1.0 in a 1 cm cell. a. I have a solution of DNA that has an transmittance of 0.15 when it is placed in a cll with a 200 um pathlength. What is the concentration of DNA in this solution (in units of g/ml)? b. Assume that this DNA is a synthetic oligo containing 25 base pairs, and that the MW of a single base pair has a molecular weight of 330. What is the molar concentration of duplex DNA molecules in the solution? c. What is the Molar absorptivity of DNA in units of L cm (mol base pair) -1 -1
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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Transcribed Image Text:One of the useful rules of thumb that you may have learned in Biochemistry, is that a
50 :g/ml solution of DNA has an absorbance of about 1.0 in a 1 cm cell.
a. I have a solution of DNA that has an transmittance of 0.15 when it is placed in a cell
with a 200 µum pathlength. What is the concentration of DNA in this solution (in units of
g/ml)?
b. Assume that this DNA is a synthetic oligo containing 25 base pairs, and that the MW
of a single base pair has a molecular weight of 330. What is the molar concentration
of duplex DNA molecules in the solution?
c. What is the Molar absorptivity of DNA in units of L cm (mol base pair) -1 -1
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