One of the useful rules of thumb that you may have learned in Biochemistry, is that a 50 :g/ml solution of DNA has an absorbance of about 1.0 in a 1 cm cell. a. I have a solution of DNA that has an transmittance of 0.15 when it is placed in a cll with a 200 um pathlength. What is the concentration of DNA in this solution (in units of g/ml)? b. Assume that this DNA is a synthetic oligo containing 25 base pairs, and that the MW of a single base pair has a molecular weight of 330. What is the molar concentration of duplex DNA molecules in the solution? c. What is the Molar absorptivity of DNA in units of L cm (mol base pair) -1 -1

One of the useful rules of thumb that you may have learned in Biochemistry, is that a 50 :g/ml solution of DNA has an absorbance of about 1.0 in a 1 cm cell. a. I have a solution of DNA that has an transmittance of 0.15 when it is placed in a cll with a 200 um pathlength. What is the concentration of DNA in this solution (in units of g/ml)? b. Assume that this DNA is a synthetic oligo containing 25 base pairs, and that the MW of a single base pair has a molecular weight of 330. What is the molar concentration of duplex DNA molecules in the solution? c. What is the Molar absorptivity of DNA in units of L cm (mol base pair) -1 -1

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter10: From Proteins To Phenotypes

Section: Chapter Questions

Problem 9QP: a. Compounds A, B, C, and D are known to be intermediates in the pathway for production of protein...

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