10. When looking at your agar plate, where are the bacteria that did not take up the plasmid during the transformation?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
icon
Related questions
Question

can you help with q 10 please. Thank you!

### Educational Crossword Puzzle

**Across:**

4. One of the components of N3 buffer that helps bind the DNA to the silica membrane.  
   **Answer:** Guanidine-HCl

6. Is beta-galactosidase translation present in blue colonies?  
   **Answer:** Yes

10. When looking at your agar plate, where are the bacteria that did not take up the plasmid during the transformation?  
   **Answer:** Lawn

11. After centrifugation, the liquid portion above the debris pellet is called this.  
   **Answer:** Supernatant

12. Precipitate of bacterial cell wall and protein.  
   **Answer:** Pellet

13. While the lysis buffer denatures DNA, the small size of DNA allows it to do this.  
   **Answer:** Elute

15. Solution that uses isopropanol to precipitate the plasmid DNA.  
   **Answer:** Sodium acetate

16. If you do not use N3 buffer, what happens to the denatured DNA?  
   **Answer:** Renature

17. Final step of plasmid purification after washing.  
   **Answer:** Elution

---

This crossword puzzle is designed to test knowledge of molecular biology techniques, specifically related to plasmid purification and transformation. Each clue highlights an important step or concept in the process, allowing learners to reinforce their understanding of how these methods are applied in a laboratory setting.
Transcribed Image Text:### Educational Crossword Puzzle **Across:** 4. One of the components of N3 buffer that helps bind the DNA to the silica membrane. **Answer:** Guanidine-HCl 6. Is beta-galactosidase translation present in blue colonies? **Answer:** Yes 10. When looking at your agar plate, where are the bacteria that did not take up the plasmid during the transformation? **Answer:** Lawn 11. After centrifugation, the liquid portion above the debris pellet is called this. **Answer:** Supernatant 12. Precipitate of bacterial cell wall and protein. **Answer:** Pellet 13. While the lysis buffer denatures DNA, the small size of DNA allows it to do this. **Answer:** Elute 15. Solution that uses isopropanol to precipitate the plasmid DNA. **Answer:** Sodium acetate 16. If you do not use N3 buffer, what happens to the denatured DNA? **Answer:** Renature 17. Final step of plasmid purification after washing. **Answer:** Elution --- This crossword puzzle is designed to test knowledge of molecular biology techniques, specifically related to plasmid purification and transformation. Each clue highlights an important step or concept in the process, allowing learners to reinforce their understanding of how these methods are applied in a laboratory setting.
Expert Solution
steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Virus
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education