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10. When looking at your agar plate, where are the bacteria that did not take up the plasmid during the transformation?

10. When looking at your agar plate, where are the bacteria that did not take up the plasmid during the transformation?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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can you help with q 10 please. Thank you!

### Educational Crossword Puzzle **Across:** 4. One of the components of N3 buffer that helps bind the DNA to the silica membrane. **Answer:** Guanidine-HCl 6. Is beta-galactosidase translation present in blue colonies? **Answer:** Yes 10. When looking at your agar plate, where are the bacteria that did not take up the plasmid during the transformation? **Answer:** Lawn 11. After centrifugation, the liquid portion above the debris pellet is called this. **Answer:** Supernatant 12. Precipitate of bacterial cell wall and protein. **Answer:** Pellet 13. While the lysis buffer denatures DNA, the small size of DNA allows it to do this. **Answer:** Elute 15. Solution that uses isopropanol to precipitate the plasmid DNA. **Answer:** Sodium acetate 16. If you do not use N3 buffer, what happens to the denatured DNA? **Answer:** Renature 17. Final step of plasmid purification after washing. **Answer:** Elution --- This crossword puzzle is designed to test knowledge of molecular biology techniques, specifically related to plasmid purification and transformation. Each clue highlights an important step or concept in the process, allowing learners to reinforce their understanding of how these methods are applied in a laboratory setting.
Transcribed Image Text:### Educational Crossword Puzzle **Across:** 4. One of the components of N3 buffer that helps bind the DNA to the silica membrane. **Answer:** Guanidine-HCl 6. Is beta-galactosidase translation present in blue colonies? **Answer:** Yes 10. When looking at your agar plate, where are the bacteria that did not take up the plasmid during the transformation? **Answer:** Lawn 11. After centrifugation, the liquid portion above the debris pellet is called this. **Answer:** Supernatant 12. Precipitate of bacterial cell wall and protein. **Answer:** Pellet 13. While the lysis buffer denatures DNA, the small size of DNA allows it to do this. **Answer:** Elute 15. Solution that uses isopropanol to precipitate the plasmid DNA. **Answer:** Sodium acetate 16. If you do not use N3 buffer, what happens to the denatured DNA? **Answer:** Renature 17. Final step of plasmid purification after washing. **Answer:** Elution --- This crossword puzzle is designed to test knowledge of molecular biology techniques, specifically related to plasmid purification and transformation. Each clue highlights an important step or concept in the process, allowing learners to reinforce their understanding of how these methods are applied in a laboratory setting.
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