Answered: On a single graph, sketch a qualitative…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

Transcribed Image Text:5. Some enzymes can catalyze substrate reactions which result in the production of H* ions. If the batch reactor is not sufficiently buffered, this can lead to a change in pH. Because pH is an important factor in optimizing enzyme activity, the reaction velocity v can be altered during the reaction by the appearance of H* as a product. On a single graph, sketch a qualitative velocity profile (velocity vs. time) for the enzymatic breakdown of the substrate Auburn by the enzyme, WarEaglease for two conditions: A. An unbuffered solution that starts at pH 9. B. A well-buffered pH 9 solution. C. A well-buffered pH 7 solution. Relative Activity (%) 100 90 80 70 60 50 40 30 20 10 0 Activity of WarEagle ase 2 3 4 7 8 9 10 11 12 13 14 PH The substrate, Auburn, is a weak base, and the reaction is carried out in a batch reactor. Assume a high substrate concentration, so depletion of [S] does not cause the reaction to slow during the experiment. The relative activity of WarEaglease as a function of pH is given in the graph above.

Expert Solution

Introduction

Activity of an enzyme is the amount of product produced per time. Relative activity of an enzyme is the amount of product produced per a fixed amount of enzyme per time.

Velocity of an enzyme catalyzed reaction is the concentration of product produced per time. Hence, relative activity is proportional to velocity. i.e. whenever relative activity decreases, velocity decreases and whenever relative activity increases, velocity increases.