Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
Of course, one cannot stick a piece of tape to each carbon atom in the actual glucose molecule. But one can do the next best thing: use radioisotopes to trace the movement of various atoms. Specifically, because carbon dioxide is a gas, using radioactive C-14 allows for the easy detection of the 14CO2 that is given off. Suppose you introduce one glucose molecule that has had all of its carbons radioactively labeled. Suppose further that each step in glycolysis and in the citric acid cycle takes one minute to complete (so glycolysis, for instance, takes ten minutes to complete, the conversion to acetyl CoA takes one minute, and the citric acid cycle takes eight minutes). Draw a plot of time (x-axis) versus radioactivity detected by a gas sensor (y-axis). Label the x-axis with enough time increments so it is clear at what minute the radioactivity was detected. Use differing peak heights to give an indication of the amount of radioactivity detected at that point

Of course, one cannot stick a piece of tape to each carbon atom in the actual glucose molecule. But one can do the next best thing: use radioisotopes to trace the movement of various atoms. Specifically, because carbon dioxide is a gas, using radioactive C-14 allows for the easy detection of the 14CO2 that is given off. Suppose you introduce one glucose molecule that has had all of its carbons radioactively labeled. Suppose further that each step in glycolysis and in the citric acid cycle takes one minute to complete (so glycolysis, for instance, takes ten minutes to complete, the conversion to acetyl CoA takes one minute, and the citric acid cycle takes eight minutes). Draw a plot of time (x-axis) versus radioactivity detected by a gas sensor (y-axis). Label the x-axis with enough time increments so it is clear at what minute the radioactivity was detected. Use differing peak heights to give an indication of the amount of radioactivity detected at that point

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question

Of course, one cannot stick a piece of tape to each carbon atom in the actual glucose
molecule. But one can do the next best thing: use radioisotopes to trace the
movement of various atoms. Specifically, because carbon dioxide is a gas, using
radioactive C-14 allows for the easy detection of the 14CO2 that is given off.
Suppose you introduce one glucose molecule that has had all of its carbons radioactively
labeled. Suppose further that each step in glycolysis and in the citric acid cycle takes one
minute to complete (so glycolysis, for instance, takes ten minutes to complete, the
conversion to acetyl CoA takes one minute, and the citric acid cycle takes eight minutes).
Draw a plot of time (x-axis) versus radioactivity detected by a gas sensor (y-axis). Label
the x-axis with enough time increments so it is clear at what minute the radioactivity
was detected. Use differing peak heights to give an indication of the amount of
radioactivity detected at that point.

Objective: To determine when during the process of respiration the carbon in glucose is released as carbon dioxide. Introduction: The process of respiration (the controlled combustion of glucose in order to gain useful energy for other metabolic processes) is a fairly complex sequence of individual chemical reaction, all mediated by enzymes. Ultimately, though, it is the process of taking a six-carbon molecule and oxidizing every carbon into CO₂. We're going to trace the fate of every carbon on one glucose molecule and see at what step it becomes CO2.
Transcribed Image Text:Objective: To determine when during the process of respiration the carbon in glucose is released as carbon dioxide. Introduction: The process of respiration (the controlled combustion of glucose in order to gain useful energy for other metabolic processes) is a fairly complex sequence of individual chemical reaction, all mediated by enzymes. Ultimately, though, it is the process of taking a six-carbon molecule and oxidizing every carbon into CO₂. We're going to trace the fate of every carbon on one glucose molecule and see at what step it becomes CO2.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps with 2 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS