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Not all of the DNA in an organism contains genes. Explain and relate in marker development to tag a gene of interest
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- Describe two of the applications for genome mapping.What is a molecular marker? Give two examples. Discuss why it isgenerally easier to locate and map molecular markers rather thanfunctional genes.DNA sequencing can help us to identify mutations withingenes. The following data are derived from an experimentin which a normal gene and a mutant gene have been sequenced:
- Give a step by step method and diagram of how reversible terminator sequencing works.The technique of fluorescence in situ hybridization (FISH) is described. This is another method for examining sequence complexity within a genome. In this method, a DNA sequence, such as a particular gene sequence, can be detected within an intact chromosome by using a DNA probe that is complementary to the sequence.For example, let’s consider the β-globin gene, which isfound on human chromosome 11. A probe complementary to theβ-globin gene binds to that gene and shows up as a brightly colored spot on human chromosome 11. In this way, researchers can detectwhere the β-globin gene is located within a set of chromosomes. Becausethe β-globin gene is unique and because human cells are diploid(i.e., have two copies of each chromosome), a FISH experimentshows two bright spots per cell; the probe binds to each copy ofchromosome 11. What would you expect to see if you used thefollowing types of probes?A. A probe complementary to the Alu sequenceB. A probe complementary to a tandem array near…Why are some genes expressed and some not? Please be as detailed as possible.
- Describe the main technique for amplifying a segment of DNA (like the one you suspect is involved in Lee’s cancer) from a complex mixture of genomic DNA. Remember that the entire human genome sequence is known. (Hint: This is a technique that is commonly used by laboratories that do genetic testing and various other applications of molecular biology.)Each of the following describes a distinctive step in a genomic technology or an experimental design. Match the term for each description. An experimental design that looks at the offspring resulting from an F2 cross, where the parental generation have different phenotypes. Then, look for correlation between offspring phenotype and genotype. The intensity of fluorescence in each spot indicates the level of gene expression. Only one fluorescent nucleotide is added, which is complementary to the position of a known SNP. Familial Design [Choose ] RNA microarray GWAS Familial Design Quantitative Genetics Genomic resequencing RNA sequencing DNA microarray Genomic resequencing <Briefly describe the creation of a transgenic mouse in your lab.
- Northern blot is an analytical technique commonly performed to study gene expression in experimental samples. Briefly illustrate and explain the steps taken to determine the presence of a specific gene in your tissue homogenate.Northern blotting, RT-PCR, and microarrays can be used to analyze gene expression. A lab studies yeast cells, comparing their growth in two different sugars, glucose and galactose. One student is comparing expression of the gene HMG2 under these two conditions. Which technique(s) could he use and why? Another student wants to compare expression of all the genes on chromosome 4, of which there are approximately 800. What technique(s) could she use and why?Give the scope of the following fields in Applied Genetics: Epigenetics Genomics Genetic Engineering Phylogenetics Metagenics take note that SCOPE is what I'm asking
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