МАРPING Xmal 611 bp 70 bp 575 bp EcoRI 673 bp 1,283 bp Xmal & EcoRI (double digest) 611 bp 62 bp 708 bp 575 bp

Q: If growth had been present on the –pGLO side of the LB/amp plate, what might have been a reason for…

A: Antibiotics are antimicrobial substance that acts against bacteria. Antibiotic resistance is the…

Q: what carries genetic information

A: Genetic information includes information about an individual's genetic tests and the genetic tests…

Q: Identify the nucleotide. CH HN 9. N' O. 0-P-OCH2 O DUMP O dGMP O DAMP O dCMP O dTMP

A: Given below is a structure of nucleotide as it contains a base, sugar & phosphate group. The…

Q: Source E coli 23.9 24.9 25.2 26.0 Yeast 31.3 32.9 18.7 17.1 Sea urchin 32.8 32.1 17.7 18.4 Rat 28.6…

A: DNA is a double-helical structure that is composed of two antiparallel strands that are wound to…

Q: What is protoplast fussion?

A: when a bacterial cell, fungal cell or a plant cell is stripped off its cell wall, the resultant…

Q: VspI BglI Bath 3나00 12800 12800 2520 1200 1200 720 720 1600

A: Restriction enzymes are defined as DNA-cutting enzymes, and each enzyme will recognize or identify…

Q: In words, describe the differences you see for each organism grown at 30 C or 37 C (i.e., amount of…

A: Micrococcus luteus are spherical (coccus) in shape and are Gram-positive. These are pigmented in…

Q: Saenger’s DNA sequencing procedure. Include the method and the main reagents used

A: Its a DNA sequencing method

Q: Explain the reason as to why Hershey and Chase did not take carbon as radioisotope but 32р as the…

A: Hershey and Chase conducted their experiment on E. coli bacteria to determine the genetic material.…

Q: samples Marker B 1,200 bp 1,000 bp 900 bp 800 bp 700 bp 600 bp s00 bp 400 bp 300 bp 200 bp 100 bp…

A: The given diagram is a representation of the process of gel electrophoresis. It is a laboratory…

Q: EcoRI alone HindIll alone Bam HI alone EcoRI+HindIll EcoRI+BamHI HindIll+ BamHI 15,000 bp 15,000 bp…

A: Answer :- In molecular biology, restriction enzymes play a pivotal role in the analysis and…

Q: give the significance/role/effect of the reagent/condition in the isolation or analysis of a…

A: Deoxy ribonucleic acid (DNA) is the genetic material of the cell which carries the information on…

Q: How many units (U) of EcoR1 did you use in the digestion? How much lambda DNA (in ugs) was digested…

A: DNA-cutting enzymes are restriction enzymes. Each enzyme recognises a single or a few target…

Q: s] Draw a picture showing one scheme for creating dilutions of E. coli cells by 104, 105, and 10-6.…

A: Serial dilution is a method that includes stepwise dilution of a substance in a sterile solvent.…

Q: Intheelectrophoresis gel results below, use the 1 kb ladder to derermine the approximate size of the…

A: Electrophoresis is a general term that describes the migration and separation of charged particles…

Q: S SimUText 2019-2020 X File Edit Go Tools Help Recycle Bin Section 1: DNA Structure NOTES 17/18…

A: The following picture depicts a nitrgenous base, a phosphate group and a deoxyribose sugar.

Q: What is the size of the DNA sample based on the gel profile below? Make sure to indicate the units.…

A: Electrophoresis is the technique to separate biomolecules depending on their…

Q: How many nanograms of DNA should we expect to be contained within the slowest band from the…

A: pGLO plasmid: pGLO is a plasmid vector mostly used for the expression of genes.  The pGLO plasmid…

Q: Comment on the boiling technique for nucleic acid extraction? Why is it done?

A: DNA extraction involves three main steps. They are, Cell disruption. DNA extraction. DNA…

Q: Why are there different spec readings (OD600) for different strains of E. coli?

A: OD value means optical density value at 600nm wavelength of light. In spectroscopy the sample means…

Q: Which of the following DNA samples has the highest melting temperature in its double- stranded form?…

A: Melting temperature of dsDNA is the temperature at which hydrogen bonds are broken and both the…

Q: A gel shows undigested lane 3000 bp while digested lane shows BS 2000 and repeat 1000. what does…

A:

Q: 29 Martian Microbe continued If the Martian Microbe replicates DNA in a fashion similar to Earth…

A: DNA replication occurs in 5'to 3'.

Q: number 5 and 5a

A: A restriction map is a map of unknown sites within a sequence of DNA and requires the use of…

Q: EcoRI alone HindIII alone Bam HI alone EcoRI + HindIll www EcoRI+ BamHI www HindlII+BamHI…

A: Restriction digestion, also known as restriction endonuclease digestion or simply restriction…

Q: Why does microbial polypeptide need fold into a 3D protein in order to become functional? Explain in…

A: There are four levels of protein structure  depending on the degree of complexity in the polypeptide…

Q: Why differential centrifugation can cause contamination in pellet?

A: Differential centrifugation is a typical process used in microbiology and cytology to separate…

Q: please explain whats going on of the following figure

A: Aptamer These are sequences of single-stranded DNA or RNA that have the ability to bind an…

Q: Reagent 1 Reaction Master Mix (3.5 rxns) 2x Polymerase mix Primer mix 5μι Water Template 5ul N/A…

A: Polymerase chain reaction (PCR) is a laboratory technique used in DNA amplification for producing…

Q: null 1 points Save Answer What is the product of translation?Transcription of part of a DNA molecule…

A: 3) DNA         CAA CAA CTT mRNA     GUU GUU GAA Protein    Val-Val-Glu From the information we can…

Q: ) whet will be will be the Amout o DNA conteing Product if melogk inG,-Phose ? metonir - 30lg DNA

A: Mitosis - equational division (2n => 2n) Meiosis - reductional division (2n => n)

Q: Why annealing, denaturation temperatures are different?  Please answer at your own words.

A: Question: Why annealing, denaturation temperatures are different?

Q: Sequence morgans experimen

A: According to the question, we have to explain the sequence of Morgan's experiment. So, let us have a…

Q: What technique was used to photograph DNA in franklins famous photo #51? A- gamma ray photography B-…

A: Imaging with gamma rays is used in nuclear medicine, as well as in court medicine. This technique…

Mitochondrial Mutations

A mitochondrion is a cellular organelle that serves as the site for cellular respiration and energy production (ATP or Adenosine Triphosphate). It is a double-membrane-bound organelle and is only present in eukaryotic cells.