Bio-123 Molecular Biology Part 1 Post/ Part 2 Pre Lab The following is a restriction map for the DNA of YIPS, a plasmid that is 5,541 base pairs (bp). The numbers indicate the location (in terms of the base pairs) of the restriction sites for each enzyme, Use the maps to answer the following questions Hindll 32 EcoR1 1/5541 Pvul 4916 Eagl 942 Apal 2035 Pvull 3247 Smal 2540 5. How many fragments would be produced if this plasmid was digested with both EcoRI and Eagl? a. What would be the size of each fragment? 6. How many fragments would be produced if this plasmid was digested with three enzymes: Hind II, Apal & Pvu lI? a. What would be the size of each fragment? 7. How many fragments would be produced if this plasmid was digested with four enzymes: Hindlll, Apol Pvul, & Pvull? a. What would be the size of each fragment? Molecular Biology Part 1-2 Post Lab V1 Short.Docx Page 4 Revised 12/2/2019

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