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Bio-123 Molecular Biology Part 1 Post/ Part 2 Pre Lab The following is a restriction map for the DNA of YIPS, a plasmid that is 5,541 base pairs (bp). The numbers indicate the location (in terms of the base pairs) of the restriction sites for each enzyme, Use the maps to answer the following questions Hindll 32 EcoR1 1/5541 Pvul 4916 Eagl 942 Apal 2035 Pvull 3247 Smal 2540 5. How many fragments would be produced if this plasmid was digested with both EcoRI and Eagl? a. What would be the size of each fragment? 6. How many fragments would be produced if this plasmid was digested with three enzymes: Hind II, Apal & Pvu lI? a. What would be the size of each fragment? 7. How many fragments would be produced if this plasmid was digested with four enzymes: Hindlll, Apol Pvul, & Pvull? a. What would be the size of each fragment? Molecular Biology Part 1-2 Post Lab V1 Short.Docx Page 4 Revised 12/2/2019

Bio-123 Molecular Biology Part 1 Post/ Part 2 Pre Lab The following is a restriction map for the DNA of YIPS, a plasmid that is 5,541 base pairs (bp). The numbers indicate the location (in terms of the base pairs) of the restriction sites for each enzyme, Use the maps to answer the following questions Hindll 32 EcoR1 1/5541 Pvul 4916 Eagl 942 Apal 2035 Pvull 3247 Smal 2540 5. How many fragments would be produced if this plasmid was digested with both EcoRI and Eagl? a. What would be the size of each fragment? 6. How many fragments would be produced if this plasmid was digested with three enzymes: Hind II, Apal & Pvu lI? a. What would be the size of each fragment? 7. How many fragments would be produced if this plasmid was digested with four enzymes: Hindlll, Apol Pvul, & Pvull? a. What would be the size of each fragment? Molecular Biology Part 1-2 Post Lab V1 Short.Docx Page 4 Revised 12/2/2019

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Bio-123 Molecular Biology Part 1 Post/ Part 2 Pre Lab The following is a restriction map for the DNA of YIPS, a plasmid that is 5,541 base pairs (bp). The numbers indicate the location (in terms of the base pairs) of the restriction sites for each enzyme, Use the maps to answer the following questions Hindll 32 EcoR1 1/5541 Pvul 4916 Eagl 942 Apal 2035 Pvull 3247 Smal 2540 5. How many fragments would be produced if this plasmid was digested with both EcoRI and Eagl? a. What would be the size of each fragment? 6. How many fragments would be produced if this plasmid was digested with three enzymes: Hind II, Apal & Pvu lI? a. What would be the size of each fragment? 7. How many fragments would be produced if this plasmid was digested with four enzymes: Hindlll, Apol Pvul, & Pvull? a. What would be the size of each fragment? Molecular Biology Part 1-2 Post Lab V1 Short.Docx Page 4 Revised 12/2/2019
Transcribed Image Text:Bio-123 Molecular Biology Part 1 Post/ Part 2 Pre Lab The following is a restriction map for the DNA of YIPS, a plasmid that is 5,541 base pairs (bp). The numbers indicate the location (in terms of the base pairs) of the restriction sites for each enzyme, Use the maps to answer the following questions Hindll 32 EcoR1 1/5541 Pvul 4916 Eagl 942 Apal 2035 Pvull 3247 Smal 2540 5. How many fragments would be produced if this plasmid was digested with both EcoRI and Eagl? a. What would be the size of each fragment? 6. How many fragments would be produced if this plasmid was digested with three enzymes: Hind II, Apal & Pvu lI? a. What would be the size of each fragment? 7. How many fragments would be produced if this plasmid was digested with four enzymes: Hindlll, Apol Pvul, & Pvull? a. What would be the size of each fragment? Molecular Biology Part 1-2 Post Lab V1 Short.Docx Page 4 Revised 12/2/2019
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