Macmillan Learning You are characterizing a new DNA polymerase. When you incubate the enzyme with [32P]-labeled DNA and no dNTPs, you observe the release of [32P]dNMPs. The addition of unlabeled dNTPs prevents this release. Explain the reactions that most likely underlie these observations. What would you expect to observe if you added pyrophosphate instead of dNTPs? The new DNA polymerase possesses generates [32P]dNDPs through 3'→5' exonuclease 5' 3' exonuclease endonuclease ✓3' 5' exonuclease 5'-3' helicase activity. The addition of pyrophosphate instead of dNTPs olymerase reaction.

Macmillan Learning You are characterizing a new DNA polymerase. When you incubate the enzyme with [32P]-labeled DNA and no dNTPs, you observe the release of [32P]dNMPs. The addition of unlabeled dNTPs prevents this release. Explain the reactions that most likely underlie these observations. What would you expect to observe if you added pyrophosphate instead of dNTPs? The new DNA polymerase possesses generates [32P]dNDPs through 3'→5' exonuclease 5' 3' exonuclease endonuclease ✓3' 5' exonuclease 5'-3' helicase activity. The addition of pyrophosphate instead of dNTPs olymerase reaction.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question 19
QUESTION 2 Within a few weeks of treatment for herpes virus with the drug isonazide, a patient's herpes virus population consists entirely of isonazide-resistant viruses. How can this result best be explained? O A. The drug caused the HIV RNA genome to change O B. The isonazide drug spontaeously converted to a form ineffective against the virus. O C HIV began making drug-resistant versions of reverse transcriptase (a viral enzyme) in response to the drug O D. None of the above
Use this information to answer the next two questions. Multiple alleles control the coat colour of rabbits. ● The dominant allele, C, produces grey colour. The Coh allele produces silver grey (chinchilla) colour when homozygous. The Challele is recessive to both the full colour allele and the chinchilla allele. ● ● The Challele produces a white colour with black extremities (Himalyan) The Ca allele is recessive to all other alleles, and produces no pigment (albino). The dominance hierarchy is C> Cch > Ch > Ca. The table below provides the possible genotypes and phenotypes for coat colour in rabbits. Notice that four genotypes are possible for full-colour but only one for albino. Phenotypes Full Colour Chinchilla Light grey Himalayan Albino JUL Genotypes CC, CCch, Cch, CCª Cch Coh Question 2 (2.5 points) In the first cross, a rabbit with the chinchilla coat colour is mated to a rabbit with a Himalavan coat colour that is known to be homozygous for this trait Calculate the 25 tv MIZA…
Question 3. Biochemistry techniques have been used for the analysis on biomolecules such as enzymes, DNA, antibodies and other molecules in biomedical engineering. b) ELISA is a plate-based assay technique, designed for detecting and quantifying substances such as peptides, proteins, antibodies and hormones. Examine two principles of ELISA. c) Gel electrophoresis is a laboratory technique, used to separate the mixture of macro- molecules such as DNA, RNA and proteins according to their molecular size. i. Analyze the characteristics of DNA molecule that affect the separation results in the gel electrophoresis. ii. Examine three common factors that are possibly affect the performance of gel electrophoresis.
Calculate the 2 possible values of x in this equation: x2 – 16x + 48 = 0, either by using the quadratic equation developed by Abu Abd-Allah ibn Musa al-Kwarizmi, or by factoring the integer values of x. A. x = 4 or 12 x = 1 or 48 x = 16 or 3 x = 24 or 2 x = 8 or 6
Calculate the 2 possible values of x in this equation: x2 – 16x + 48 = 0, either by using the quadratic equation developed by Abu Abd-Allah ibn Musa al-Kwarizmi, or by factoring the integer values of x. A. x = 4 or 12 x = 1 or 48 x = 16 or 3 x = 24 or 2 x = 8 or 6
Question 20 You are studying a species of butterfly whose caterpillars produce different toxins based on the genotype of the Tox locus. Alleles E and N are codominant. Individuals homozygous for E are emetic (cause vomiting), while caterpillars homozygous for N produce a neurotoxin. Heterozygotic caterpillars produce a toxin with both qualities. At the Bocas del Toro research station in Panama, you counted 45 neurotoxic caterpillars, 57 emetic caterpillars, and 82 caterpillars with both toxic qualities. Using the Hardy-Weinberg equilibrium equation, you calculated the expected genotypic frequencies: NN= 0.218, NE= 0.498, EE= 0.284. Is this population in Hardy-Weinberg Equilibrium? Why/why not? A) No, because the X^2 test statistic is greater than 3.84. B) No, because the genotype frequencies do not equal 1. c) Yes, because the X^2 test statistic is greater than 3.84. D Yes, because the genotype frequencies are approximately equal to 1. E) No, because the X^2 test statistic is less than…