Lysosomal hydrolases contain sorting signals that mark these proteins for modification with mannose 6-phosphate. Lysosomal sorting signals are unusual in that they are not peptides attached to the termini of the proteins. Instead, lysosomal sorting signals are “patches” on the folded proteins. Such a signal patch may include amino acids that are distant from one another in the linear polypeptide chain, but are brought close together by protein folding. When cell biologists identify a sorting signal, they confirm that the signal is necessary and sufficient. Suppose that you have identified a putative signal patch on a lysosomal hydrolase. How would you test whether this signal is necessary and sufficient to mark the protein for modification with mannose 6-phosphate?

Lysosomal hydrolases contain sorting signals that mark these proteins for modification with mannose 6-phosphate. Lysosomal sorting signals are unusual in that they are not peptides attached to the termini of the proteins. Instead, lysosomal sorting signals are “patches” on the folded proteins. Such a signal patch may include amino acids that are distant from one another in the linear polypeptide chain, but are brought close together by protein folding. When cell biologists identify a sorting signal, they confirm that the signal is necessary and sufficient. Suppose that you have identified a putative signal patch on a lysosomal hydrolase. How would you test whether this signal is necessary and sufficient to mark the protein for modification with mannose 6-phosphate?

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter9: Gene Expression And Gene Regulation

Section: Chapter Questions

Problem 18QP: The following is a portion of a protein: met-trp-tyr-arg-gly-pro-thr-Various mutant forms of this...

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