Luther found a vial containing an unknown lipid sample. He separatedthe different lipids using gel filtration chromatography anddetermined the identities of the different lipid eluents using normalphase TLC. Help Luther complete the table below. Report thedistance travelled of eluents and solvent in two decimal places andthe Rf in four decimal places. For the identities of the lipids, refer tothe provided pool of chemical structures and write only the lettercode.EluentsDistanceTravelled (cm)RfIdentityEluent A(1)0.5541(5)Eluent B5.90.3758(6)Eluent C13.25(3)(7)Eluent D(2)0.2325(8)Solvent front, cm(4)POOL OF CHEMICAL STRUCTURES:LETTERCODEOFFGUNEARTHMIXHOSTRUCTUREH

The dimensionless number is used in TLC to measure a substance's relative movement concerning the…

Answered: Luther found a vial containing an…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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NOTE: translucent (positive for fats) and opaque (negative for fats) To test for lipids, when we put one drop of corn oil on a paper towel (positive control): what opacity (translucent or opaque) did you observe in the paper towel? Does that mean the substance is present or absent? To test for lipids, when we put one drop of distilled water on a paper towel (negative control): what opacity (translucent or opaque) did you observe in the paper towel? Does that mean the substance is present or absent? What is the purpose of having controls when testing for lipids in this experiment?
A paste of starch was tested using Benedict’s test, the Iodine Test, and Fehling’s Test. Before hydrolysis, Benedict’s test and Fehling's test results were both negative while Iodine’s test is positive. After adding 0.5 mL of conc. HCl to the paste, another 5 drops of NaOH were added and was tested using Fehling’s, Benedict’s Test, and Iodine Tests. What would be the theoretical result of the three different tests? Explain the chemical reaction that happened and the principle/mechanism behind the reactions.
The pH scale shows availability of reactive hydrogen ions (H+) in a liquid. The scale is logarithmic, so milk has 10 times as many H+ ions as pure water, for a given volume. How many more H+ ions does soda pop have compared to pure water? Write the number only.
If skin cleaning solution is to be diluted 1 teaspoon to 64 ounces of water, how much water would need to be added to a container containing 1/4 teaspoon of skin cleaning solution?
Study the following urine test results and complete Tables 1 and Write “+” signs in the appropriate boxes of Table 2 to indicate different medical problems. Tests A B C D E Leukocyte Nitrite Protein pH Blood Specific Gravity Ketone Glucose Table 2: Medical Problems Indicated by Urinalysis Results Diagnoses A B C D E Urinary Tract Infection Diabetes Kidney Stone Liver Disease Kidney Disease Unclean catch
List at least three test reactions for lipids. Describe its principle and its positive result
For the questions below be sure to show your work as well as the answer to the question. In this week's lab you made 100ml of 10x PBS by dissolving 8 g NaCl, 1.2g Na₂HPO4 and 0.2 g KH₂PO4 in water. You then adjusted the pH to 7.0. A) How many mg of KH₂PO4 did you weigh out? B) How many liters of 10x PBS did you make? C) What is the final concentration of NaCl in your solution? (The molecular weight of NaCl is 58.44 g/mol) D) What is the final concentration of Na₂HPO4 in your solution? (The molecular weight of Na₂HPO4 is 141.96 g/mol) E) What is the final concentration of KH₂PO4 in your solution? (The molecular weight of KH₂PO4 is 136.1 g/mol) F) What is the hydrogen ion concentration of your solution (pH=7.0)? G) If the initial pH of your solution was 6.29 would you need to add an acid or a base to adjust the pH of a solution to pH 7.0? 10x PBS is a concentrated stock solution that is used to make more dilute solutions of PBS. Starting with your 10xPBS stock solution, how would you…

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