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For the questions below be sure to show your work as well as the answer to the question. In this week's lab you made 100ml of 10x PBS by dissolving 8 g NaCl, 1.2g Na₂HPO4 and 0.2 g KH₂PO4 in water. You then adjusted the pH to 7.0. A) How many mg of KH₂PO4 did you weigh out? B) How many liters of 10x PBS did you make? C) What is the final concentration of NaCl in your solution? (The molecular weight of NaCl is 58.44 g/mol) D) What is the final concentration of Na₂HPO4 in your solution? (The molecular weight of Na₂HPO4 is 141.96 g/mol) E) What is the final concentration of KH₂PO4 in your solution? (The molecular weight of KH₂PO4 is 136.1 g/mol)

For the questions below be sure to show your work as well as the answer to the question. In this week's lab you made 100ml of 10x PBS by dissolving 8 g NaCl, 1.2g Na₂HPO4 and 0.2 g KH₂PO4 in water. You then adjusted the pH to 7.0. A) How many mg of KH₂PO4 did you weigh out? B) How many liters of 10x PBS did you make? C) What is the final concentration of NaCl in your solution? (The molecular weight of NaCl is 58.44 g/mol) D) What is the final concentration of Na₂HPO4 in your solution? (The molecular weight of Na₂HPO4 is 141.96 g/mol) E) What is the final concentration of KH₂PO4 in your solution? (The molecular weight of KH₂PO4 is 136.1 g/mol)

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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For the questions below be sure to show your work as well as the answer to the question. In this week's lab you made 100ml of 10x PBS by dissolving 8 g NaCl, 1.2g Na₂HPO4 and 0.2 g KH₂PO4 in water. You then adjusted the pH to 7.0. A) How many mg of KH₂PO4 did you weigh out? B) How many liters of 10x PBS did you make? C) What is the final concentration of NaCl in your solution? (The molecular weight of NaCl is 58.44 g/mol) D) What is the final concentration of Na₂HPO4 in your solution? (The molecular weight of Na₂HPO4 is 141.96 g/mol) E) What is the final concentration of KH₂PO4 in your solution? (The molecular weight of KH₂PO4 is 136.1 g/mol) F) What is the hydrogen ion concentration of your solution (pH=7.0)? G) If the initial pH of your solution was 6.29 would you need to add an acid or a base to adjust the pH of a solution to pH 7.0? 10x PBS is a concentrated stock solution that is used to make more dilute solutions of PBS. Starting with your 10xPBS stock solution, how would you make the following solutions? 100 mls of 1xPBS 30 mls of 3xPBS 11 of 0.1xPBS
Transcribed Image Text:For the questions below be sure to show your work as well as the answer to the question. In this week's lab you made 100ml of 10x PBS by dissolving 8 g NaCl, 1.2g Na₂HPO4 and 0.2 g KH₂PO4 in water. You then adjusted the pH to 7.0. A) How many mg of KH₂PO4 did you weigh out? B) How many liters of 10x PBS did you make? C) What is the final concentration of NaCl in your solution? (The molecular weight of NaCl is 58.44 g/mol) D) What is the final concentration of Na₂HPO4 in your solution? (The molecular weight of Na₂HPO4 is 141.96 g/mol) E) What is the final concentration of KH₂PO4 in your solution? (The molecular weight of KH₂PO4 is 136.1 g/mol) F) What is the hydrogen ion concentration of your solution (pH=7.0)? G) If the initial pH of your solution was 6.29 would you need to add an acid or a base to adjust the pH of a solution to pH 7.0? 10x PBS is a concentrated stock solution that is used to make more dilute solutions of PBS. Starting with your 10xPBS stock solution, how would you make the following solutions? 100 mls of 1xPBS 30 mls of 3xPBS 11 of 0.1xPBS
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