Jungkook was tasked to ensure that an enzyme responsible for keeping her species from extinction remains biologically active. To do so, she needs to store it in a buffer solution with pH 6.50. Unfortunately, only three acids with their corresponding conjugate base salts are available in their laboratory as shown below. Ка Conjugate Base Salt Benzoic acid, CoH;COOH 6.25 x 10s NaCsHsCOO Boric acid, H3BO, 5.81 x 107 NaH;BO, Hydrogen cyanide, HCN 6.20 X 1010 NaCN 1. In what buffer system will Jungkook store the enzyme? 2. Write the dissociation reaction involved in the buffer system. 3. Write the pertinent chemical equations to illustrate what happens upon the addition of small amounts of (a) strong base and (b) strong acid.
Jungkook was tasked to ensure that an enzyme responsible for keeping her species from extinction remains biologically active. To do so, she needs to store it in a buffer solution with pH 6.50. Unfortunately, only three acids with their corresponding conjugate base salts are available in their laboratory as shown below. Ка Conjugate Base Salt Benzoic acid, CoH;COOH 6.25 x 10s NaCsHsCOO Boric acid, H3BO, 5.81 x 107 NaH;BO, Hydrogen cyanide, HCN 6.20 X 1010 NaCN 1. In what buffer system will Jungkook store the enzyme? 2. Write the dissociation reaction involved in the buffer system. 3. Write the pertinent chemical equations to illustrate what happens upon the addition of small amounts of (a) strong base and (b) strong acid.
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 7.6P
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![Jungkook was tasked to ensure that an enzyme responsible for keeping her species from extinction
remains biologically active. To do so, she needs to store it in a buffer solution with pH 6.50.
Unfortunately, only three acids with their corresponding conjugate base salts are available in their
laboratory as shown below.
Ка
Conjugate Base
Salt
Benzoic acid, CH;COOH
6.25 x 105
NaCsHsCOO
Boric acid, H3BO3
5.81 x 107
NaH2BO3
Hydrogen cyanide, HCN
6.20 X 10'10
NaCN
1. In what buffer system will Jungkook store the enzyme?
2. Write the dissociation reaction involved in the buffer system.
3. Write the pertinent chemical equations to illustrate what happens upon the addition of small
amounts of (a) strong base and (b) strong acid.
4. Calculate the relative amounts of the buffer components if you were to prepare 500 mL of an
80 mM buffer pH 6.50 using 100 mM stock solutions of its components.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F91b08917-4f48-47cf-b04e-cce0864c0ab2%2F079b9cd2-4282-4d97-be0e-6f94e667d98c%2Fqy42229_processed.png&w=3840&q=75)
Transcribed Image Text:Jungkook was tasked to ensure that an enzyme responsible for keeping her species from extinction
remains biologically active. To do so, she needs to store it in a buffer solution with pH 6.50.
Unfortunately, only three acids with their corresponding conjugate base salts are available in their
laboratory as shown below.
Ка
Conjugate Base
Salt
Benzoic acid, CH;COOH
6.25 x 105
NaCsHsCOO
Boric acid, H3BO3
5.81 x 107
NaH2BO3
Hydrogen cyanide, HCN
6.20 X 10'10
NaCN
1. In what buffer system will Jungkook store the enzyme?
2. Write the dissociation reaction involved in the buffer system.
3. Write the pertinent chemical equations to illustrate what happens upon the addition of small
amounts of (a) strong base and (b) strong acid.
4. Calculate the relative amounts of the buffer components if you were to prepare 500 mL of an
80 mM buffer pH 6.50 using 100 mM stock solutions of its components.
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