The trypsin enzyme is able to hydrolyze a peptide substate at the carboxyl side of an Arg or Lys residue. However, such a reaction can be also influenced by the amino acid residue that follows Arg or Lys. Given the Michaelis-Menton plots obtained for two substrates (substrate A: Ser-Val- Arg-Pro; substrate B: Ser-Val-Arg-Phe), the Km of the enzyme is higher for: 20 Bubsrate Assume that these curves do not reach the same Smit. Subatrate (micromoles/itersecond) Aysojan en

Biochemistry
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ISBN:9781319114671
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Chapter1: Biochemistry: An Evolving Science
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The trypsin enzyme is able to hydrolyze a peptide substate at the carboxyl
side of an Arg or Lys residue. However, such a reaction can be also
influenced by the amino acid residue that follows Arg or Lys. Given the
Michaelis-Menton plots obtained for two substrates (substrate A: Ser-Val-
Arg-Pro; substrate B: Ser-Val-Arg-Phe), the Km of the enzyme is higher
for:
20
Substrate A
Assume
that these
curves do
not reach
the same
limit.
15
Substrate E
0.001
0.002
0.003
(Substrate) (molar)
Inconclusive
Substrate B
Both Substrate A and B
Intitial velocity
(micromoles/literisecond)
Transcribed Image Text:The trypsin enzyme is able to hydrolyze a peptide substate at the carboxyl side of an Arg or Lys residue. However, such a reaction can be also influenced by the amino acid residue that follows Arg or Lys. Given the Michaelis-Menton plots obtained for two substrates (substrate A: Ser-Val- Arg-Pro; substrate B: Ser-Val-Arg-Phe), the Km of the enzyme is higher for: 20 Substrate A Assume that these curves do not reach the same limit. 15 Substrate E 0.001 0.002 0.003 (Substrate) (molar) Inconclusive Substrate B Both Substrate A and B Intitial velocity (micromoles/literisecond)
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