Jean is the mother of Dani, Lelia, Jose and Pam. Alex could be the father, You use PCR analyze 2 genes known to show different numbers of repeated sequences. After gel electrophoresis, you get the following result Gene 1 Jean Alex Jean Alex Dani Dani Leila Jose Based on these results Alex must be the father of Pam Leila Jose Pam Alex could be the father of Pam Alex can not be the father of Pam Pam
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- Please answer Discuss if you could perform the PCR and then agarose gel electrophoresis using a coding gene instead of a VNTRNikolas is investigating the presence of four different genes (A, B, C and D) in Ladybugs using a PCR technique illustrated below (Figure 2). Deduce FOUR (4) possible reasons why this method is utilized rather than the conventional PCR technique. 422 318 275 232 181 109 Marker 01 PCR with all four primer pairs in a single tube 371 275 202 141 Figure 2Regarding STR markers used in forensic science. Tick all the correct statements: no correct statement the PCR primers used to amplify STRs are located in the repeat units PCR primers to amplify STRs are located on both sides of the repeat units the PCR primers used to amplify STRs are coupled to a fluorochrome which is essential for the detection of amplicons they are absent from the gonosomes the allelic frequencies of STR markers vary according to the ethnicity of the individuals genotyped they are present homogeneously throughout the nuclear genome
- The primers used in PCR are double stranded DNA oligosaccharides. True False2 of 11 Identify the incorrect statements O Taq polymerase can incorporate nucleotides at a rate of -60bp per second at 70°C It is possible to re-amplify PCR products to obtain a higher yield of the target Steps in PCR are typically repeated (cycled) 25-35 times The maximum PCR product length that Taq polymerase can typically amplify is approximately 10 kbp V Extension is the second step in PCRCan you explain thie each of the statement given i dont really understand the dna recombinant is used as a molecular cloning and application for recombinant dna
- BamHI KpnI SpeI XhoI PatI HindIII 400 500 200 300 700 NotI 75 2580 ECORI Frog DNA BamHI 575 KpnI HindIII 625 2150 HindIII 700 PstI clal 750 РКАВОО 2700bp 915 1900 SpeI AluI 1050 1525 BamHI ori You wish to make a recombinant DNA molecule that will contain one piece of pKABOO vector DNA and one piece of frog DNA so that you can clone a segment of frog DNA. You want cells containing your recombinant plasmid to be amp' and tet and you want to use enzymes that cut within the insertional marker gene. (Note that tet means that there is no functional tet gene in the plasmid.) Be sure that your plasmid has the ability to replicate autonomously in a bacterial cell. You do not have to include the entire frog DNA given below in your recombinant plasmid. Restriction enzymes would be used to clone segment of frog DNA The size of the recombinant plasmid is bp. The recombinant plasmid when transformed into E. coli confers resistance to which of the following antibiotics: Oampicillin only Otetracycline…If the EcoRI/HindIII (stuffer) fragment cut from the vector is religated back into the vector, the M13F/M13R primers will: O Amplify the stuffer fragment because the primers are designed to complement the plasmid DNA O Not amplify the stuffer fragment because the primers are designed to complement human gDNAIdentify if the following statements are true or false. Quantitative PCR is also known as the real-time PCR Real-time PCR method uses fluorescent dyes such as acridine orange
- Can you please check my answer and see if it is correct. Question: In addition to master mix, what else must be added to each PCR tube? Why? Answer: In addition to master mix, the primers must be added to the PCR tubes. Each tube is also reserved for one individual’s specific section of DNA. So not only do primers needed to be added that are made specifically for the person’s DNA, but the DNA itself from the suspect needs to be added to the PCR tubes (DNA template strand). We also must have a control which contains sterile water, and one PCR tube reserved for DNA from the crime scene.The following figure shows the FBI-style analysis of the genomic DNA of 10 people (1-10), and also of hair found at a crime scence left by the murderer [***]. This analysis involves the PCR amplification of SSR loci, each from a different (nonhomologus) chromosome. The PCR primers are for each SSR locus are labeled with a unique fluorescent molecule. Some bands are thicker because relatively more of the corresponding PCR product was obtained. The figure has dots aligned on both sides to help you find the crucial bands; it will help to use a straight-edge as a guide. The numbers at right are the total number of copies of the SSR locus among the population of 11 samples. Are any of individuals 1-10 probable relatives of the murderer? If so, identify this person and describe the degree of relationship to the criminal.The following figure shows the FBI-style analysis of the genomic DNA of 10 people (1-10), and also of hair found at a crime scence left by the murderer [***]. This analysis involves the PCR amplification of SSR loci, each from a different (nonhomologus) chromosome. The PCR primers are for each SSR locus are labeled with a unique fluorescent molecule. Some bands are thicker because relatively more of the corresponding PCR product was obtained. The figure has dots aligned on both sides to help you find the crucial bands; it will help to use a straight-edge as a guide. The numbers at right are the total number of copies of the SSR locus among the population of 11 samples. Are any of the loci on the Y chromosome? If so, identify this (these) locus (loci) by color.