iven the following protein mixture, answer the following question a. using hydrophobic interaction chromatography, what is the protein that will be eluted first| b. using affinity chromatography the protein that will be eluted LAST in an antigen containing column is c. the protein with the least migration in SDS-PAGE is?

Q: The loopy polypeptide segments at the very top of the structure shown are the segments that actually…

A: Antibodies and antibody-derived molecules have authenticated themselves as the main protein-based…

Q: an antibody the variable regions form the __________________

A: The antibody is a glycoproteins and is also called as immunoglobulin (Ig). The B cells are…

Q: In (b), why would it be more efficient to use labeled anti-human IgG rather than label the patient’s…

A: In direct method of antigen detection, the primary antibody is directly attached to a label. It…

Q: Draw the general structure of an antibody and list the different classes as well as their abundance

A: The antibody or immunoglobulins (Ig) are circulating glycoprotein molecules secreted by…

Q: Consider a pencil-shaped protein with two epitopes, Y (the “eraser” end) and Z (the “point” end).…

A: Antibodies are glycoproteins that bind to specific epitopes located on antigen surfaces and cause…

Q: Compare and contrast ELISA and western blotting (immunoblotting) by placing the phrases to the…

A: Both ELISA & Western Blot are laboratory techniques which are used for the detection of specific…

Q: Why does the antibody titer determination use twofold dilutions ofthe antiserum rather than 10-fold…

A:

Q: The scale bar in panels (a) and (d) in this image is 50 um (micrometeraj. Question: Which of the…

A: Microscope   A device use to visualise non visible extremely small microorganisms.

Q: Which domain of antibody defines its type and which domain is for specificity against antigen. Also…

A: Antibody is also known as immunoglobulin is a Y-shaped protein. They helps in providing immunity to…

Q: A) What  additional reagents would you need to perform immunostaining of frozen sections of mouse…

A: The process used here is immunohistochemistry followed by immunostaining. Immunohistochemical…

Q: Identify the highlighted part of the antibody

A: Immunity is the most talked-about term in this covid pandemic, it defines to be the body's mechanism…

Q: You are interested in performing indirect immunofluorescence light microscopy to observe the…

A: Immunofluorescence light microscopy is a laboratory technique that uses fluorescently labeled…

Q: An injection contains 5 million international units (MIU) of interferon alpha-n3  (ALFERON N)…

A: Interferon alpha n3 is a purified form of human interferon used to stimulate innate antiviral…

Q: Biochemists tend to classify protein structures into four groups: mostly alpha, mostly beta, mixed…

A: Immunoglobulin is a specialized protein that helps in immunity. This is a Y-shaped tetramer…

Q: In this chapter, we described co-immunoprecipitation as a method for identifying binding partners to…

A: Co-immunoprecipitation (Co-IP) is a laboratory technique used to identify and characterize…

Q: Observe the structure of antibodies below that has four protein subunits. Explain why we only see…

A: SDS-PAGE separates proteins primarily by mass because the ionic detergent SDS denaturates and binds…

Q: In the table below, T cell proliferation was measured after 4 days of incubation of T cells, APCs,…

A: T lymphocyte is a kind of leukocyte or white blood cell) that plays an important role in the immune…

Q: Before the mechanism for generating antibody diversity had been established, a mechanism based on…

A: Any abnormal condition that hinders normal body functioning is considered a disease. Nutrient…

Q: Suppose you were instructed to make two antiserum solutions: The first is identical to what you used…

A: Precipitation test is a antigen and antibody reaction in a given test solution to form a insoluble…

Q: Cell line A was cultured in the absence and presence of KB9520 or PPT. You then lyse the cells and…

A: Estrogen receptors (ERs) are a family of transcription factors that play a critical role in a…

Q: The vaccine for Haemophilus influenzae type b is called a conjugate vaccine. It is composed of the…

A: The Hib conjugate vaccine is designed to provide immunity against infections caused by the bacterium…

Q: Labs that do a lot of Western blots often have hundreds of primary bodies but only two or three…

A: Western blotting involves binding of a primary antibody to the target antigen followed by binding of…

Q: Have a look at the results in this SDS-PAGE gel. A MW ladder is shown. In the "control" lane, a…

A: In analysis of gel electrophoresis data, the band represents qualitative data, i.e. whether the…

Q: The following reactions are all antigen(Ag)-antibody(Ab) complexes. Match the description with the…

A: Antibody-antigen reactions like precipitin and agglutination involve antibodies binding to soluble…

Q: You have 4 ml of an antigen solution, how would you prepare a 3-fold dilution series such that you…

A: Dilution means decreasing the concentration. 3-fold dilution means decreasing the concentration 3…

Q: Mangaba, the laboratory technician, is interested in determining narcotics in saliva at this…

A: Narcotics are a class of highly strong medications. They are frequently administered to treat pain.…

Q: How would you prepare a 1:350 dilution of secondary antibody in a total volume of 3mL

A: Both secondary and primary antibodies are used in Western blotting and many other procedures to…

Q: GenAlex Medical, a leading manufacturer of medical laboratory equipment, is designing a new…

A: Introduction  The immune system uses antibodies, often referred to as immunoglobulins, to recognise…

Q: dentify the parts of the antibody and match the description of the parts of the antibody with their…

A: Antibodies are the antigen-binding glycoproteins which are synthesized exclusively by B-cells and in…

Q: Mr. Mangaba, the laboratory technician, is interested in determining narcotics in saliva at this…

A: Introduction  The treatment of disease by immune system activation or suppression is known as…

Q: how would you prepare a 3-fold dilution series

A: Serial dilution is a step by step dilution process in which the the solution gets more and more…

Q: Why does the eliza with two antibodies incubate after adding the substrates?

A: ELISA are a type of immunoassay that are commonly used to quantify the specific target within a…