iven the following protein mixture, answer the following question a. using hydrophobic interaction chromatography, what is the protein that will be eluted first| b. using affinity chromatography the protein that will be eluted LAST in an antigen containing column is c. the protein with the least migration in SDS-PAGE is?

Biochemistry
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Chapter1: Biochemistry: An Evolving Science
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Given the following protein mixture, answer the following question
a. using hydrophobic interaction chromatography, what is the protein that will be eluted first|
b. using affinity chromatography the protein that will be eluted LAST in an antigen containing column is
c. the protein with the least migration in SDS-PAGE is?

Affinity
Size
IProtein
%Hydrophobicity!
to
(kDa);
Molecule
A
300
75
В
85
Antigen
60
22
45
-.
--------
Transcribed Image Text:Affinity Size IProtein %Hydrophobicity! to (kDa); Molecule A 300 75 В 85 Antigen 60 22 45 -. --------
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