2. Is FRAP useful for studying the movement of peripheral membrane proteins on themembrane? Justify your answer.

Introduction Plasma membrane is the outermost covering of the animal cell. The Plasma membrane is…

Answered: Is FRAP useful for studying the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Your research advisor would like you to measure the movement of a protein X using FRAP. The results of your FRAP experiment are shown below. What can you conclude? Fluorescence Intensity (Arbitrary Units) Time (Seconds) Time point of exposure to an intense laser Protein X appears to be completely immobilized in the membrane Protein X appears to be partially immobilized in the membrane You cannot make predictions about the mobility of Protein X with these data Protein X appears to be completely mobile in the membrane
Provide examples and describe basic structure/function of plasma membrane components– Phospholipids (Which is charged, what is the functional significance? Are they preferentially located on cytosolic or extracellular lipid monolayer? What is the functional significance of plasma membrane asymmetry?)– Describe phosphatidylserine signaling– Cholesterol: how does it maintain optimal fluidity?– Glycolipids– Proteins
An additional dark control is recommended for membrane analysis, one that includes the membrane disrupting agent. Why might you want this added control reaction?
Do you think there are concentration gradients? If so, for which ions are there concentration gradients? Specify whether the concentration values of these ions are higher or lower inside the cell.
By providing examples, explain what roles molecular motors play in the transport of vesicles and the intercellular organisation of organelles.
Is this statement true or false? exlain why
What type of signal sequence(s) would be used to create the ACE2 (type 1) integral membrane protein?
Integral membrane proteins... Choose all that could apply are bound to the membrane by only interacting with the phospholipid's polar head O contain many amino acids with hydrophobic residues O contain alpha-helical membrane spanning domains O would not be digested by trypsin in a permeabilized cell O interact with the phospholipid core of the phospholipid bilayer
Consider the artificial cell experiment below: Lactose = 2.0 M Ca+ = 0.4 M The ion channels in the membrane will allow Ca+ to pass freely, but nothing else. The starting conditions for the experiment are shown. Lactose = 0.1 M Ca+ = 3.0 M Rewrite the following statement to make it correct: "Eventually, there will be a net movement of water out of the cell because the inside of the cell is hypotonic compared to the environment." Edit View Insert Format Tools Table 12pt ✓ Paragraph
1. (a) In class thus far, we have focused our membrane transport energetics discussions on the transfer of K+ ions. Of course, in the cell there are other ions that contribute to the overall resting membrane potential (Autotal). To estimate the overall resting membrane potential for the predominant ions present in the cell, we must first calculate the individual resting membrane potential. Using the Nernst equation discussed in class, and the values provided below, calculate A for each ion. lon K+ Na+ Ca²+ CI- [ion] outside cell 6 mM 145 mM 4 mM 90 mM [ion] inside cell 145mM 8 mM 0.001 mM 6 mM
From the image provided. Do you think there are concentration gradients? If so, for which ions are there concentration gradients? Specify whether the concentration values of these ions are higher or lower inside the cell.

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Is FRAP useful for studying the movement of peripheral membrane proteins on the membrane? Justify your answer.